• Western blot analysis of extracts of various cell lines, using Alpha-2-Macroglobulin (Alpha-2-Macroglobulin (A2M) ) antibody (STJ22453) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-A2M antibody (1155-1474) (STJ22453)

SKU:
STJ22453

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-A2M (1155-1474) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: A2M
Gene ID: 2
Uniprot ID: A2MG_HUMAN
Immunogen Region: 1155-1474
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1155-1474 of human Alpha-2-Macroglobulin (Alpha-2-Macroglobulin (A2M) ) (NP_000005.2).
Immunogen Sequence: ALAGNQDKRKEVLKSLNEEA VKKDNSVHWERPQKPKAPVG HFYEPQAPSAEVEMTSYVLL AYLTAQPAPTSEDLTSATNI VKWITKQQNAQGGFSSTQDT VVALHALSKYGAATFTRTGK AAQVTIQSSGTFSSKFQVDN NNRLLLQQVSLPELPGEYSM KVTGEGCVYLQTSLKYNILP EKEEFPFALGVQTLPQTCDE PKAHTSFQISLSVSYTGSRS ASNMAIVDVKMVSGFIPLK
Tissue Specificity Secreted in plasma.
Function Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Protein Name Alpha-2-Macroglobulin
Alpha-2-M
C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 5
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-140837
Reactome: R-HSA-1474228
Reactome: R-HSA-8963896
Cellular Localisation Secreted
Alternative Antibody Names Anti-Alpha-2-Macroglobulin antibody
Anti-Alpha-2-M antibody
Anti-C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 5 antibody
Anti-A2M antibody
Anti-CPAMD5 antibody
Anti-FWP007 antibody

Information sourced from Uniprot.org

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