• Western blot analysis of extracts of various cell lines, using AARS2 antibody (STJ110136) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-AARS2 antibody (841-985) (STJ110136)

SKU:
STJ110136

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AARS2 (841-985) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AARS2
Gene ID: 57505
Uniprot ID: SYAM_HUMAN
Immunogen Region: 841-985
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 841-985 of human AARS2 (NP_065796.1).
Immunogen Sequence: RRELLATVKMLQRRANTAIR KLQMGQAAKKTQELLERHSK GPLIVDTVSAESLSVLVKVV RQLCEQAPSTSVLLLSPQPM GKVLCACQVAQGAMPTFTAE AWALAVCSHMGGKAWGSRVV AQGTGSTTDLEAALSIAQTY ALSQL
Function Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
Protein Name Alanine--Trna Ligase - Mitochondrial
Alanyl-Trna Synthetase
Alars
Database Links Reactome: R-HSA-379726
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Alanine--Trna Ligase - Mitochondrial antibody
Anti-Alanyl-Trna Synthetase antibody
Anti-Alars antibody
Anti-AARS2 antibody
Anti-AARSL antibody
Anti-KIAA1270 antibody

Information sourced from Uniprot.org

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