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Anti-Beta Actin antibody

[STJ91464] Download PDF Print Data Sheet
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Product name Anti-Beta Actin antibody
Short Description Rabbit polyclonal antibody to beta actin.
Description Beta actin is a member of the actin protein family and is encoded by the ACTB gene. It localises to the cytoplasm where it acts as a cellular cytoskeleton, and is also involved in pathways at the blood-brain barrier, immune cell transmigration, regulation of actin cytoskeleton by Rho GTPases and development Slit-Robo signalling.
Applications ELISA, IHC-p, WB
Dilution range WB 1:1000-1:4000
IHC 1:100-1:300
ELISA 1:20000
Specificity Beta actin polyclonal antibody detects endogenous levels of beta actin protein.
Protein Name Actin, cytoplasmic 1
Beta-actin
Actin, cytoplasmic 1, N-terminally processed
Immunogen Synthesized peptide derived from human beta actin.
Immunogen Region N-terminal
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Mouse, Rat, Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 60
Gene Symbol ACTB
Molecular Weight 42 kDa
Database Links HGNC:132
OMIM:102630
Alternative Names Actin, cytoplasmic 1 antibody
Beta-actin antibody
Actin, cytoplasmic 1, N-terminally processed antibody
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Post-translational Modifications MICALs (MICAL1, MICAL2 or MICAL3) will oxidise amino acids Met-44 and Met-47 to form methionine sulfoxide, which in turn promotes the depolymerisation of actin filaments. MICAL1 and MICAL2 will produce the (R)-S-oxide form, which is reverted by MSRB1 and MSRB2, which promote actin repolymerization.
Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 during infection. Bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins. Toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.
Cellular Localization Cytoplasm, cytoskeleton
Swiss-Prot Key P60709_HUMAN
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