• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using BUB1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-BUB1 antibody (251-300 aa) (STJ91912)

SKU:
STJ91912

Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mitotic checkpoint serine/threonine-protein kinase BUB1 (251-300 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BUB1
Gene ID: 699
Uniprot ID: BUB1_HUMAN
Immunogen Region: 251-300 aa
Specificity: Bub1 Polyclonal Antibody detects endogenous levels of Bub1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human BUB1 at the amino acid range 251-300
Post Translational Modifications Upon spindle-assembly checkpoint activation it is hyperphosphorylated and its kinase activity toward CDC20 is stimulated. Phosphorylation at Thr-609 is required for interaction with PLK1, phosphorylation at this site probably creates a binding site for the POLO-box domain of PLK1, thus enhancing the PLK1-BUB1 interaction. Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1.
Function Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Promotes the centromeric localization of TOP2A. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.
Protein Name Mitotic Checkpoint Serine/Threonine-Protein Kinase Bub1
Hbub1
Bub1a
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Nucleus
Chromosome
Centromere
Kinetochore
Nuclear In Interphase Cells
Accumulates Gradually During G1 And S Phase Of The Cell Cycle
Peaks At G2/M
And Drops Dramatically After Mitosis
Localizes To The Outer Kinetochore
Kinetochore Localization Is Required For Normal Mitotic Timing And Checkpoint Response To Spindle Damage And Occurs Very Early In Prophase
Aurkb
Knl1 And Incenp Are Required For Kinetochore Localization
Alternative Antibody Names Anti-Mitotic Checkpoint Serine/Threonine-Protein Kinase Bub1 antibody
Anti-Hbub1 antibody
Anti-Bub1a antibody
Anti-BUB1 antibody
Anti-BUB1L antibody

Information sourced from Uniprot.org

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