• Western blot analysis of extracts of various cell lines, using HP1 alpha/HP1 alpha/CBX5 antibody (STJ22922) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
  • Western blot analysis of extracts from normal (control) and HP1 alpha/HP1 alpha/CBX5 rabbit polyclonal antibody knockout (KO) 293T cells, using HP1 alpha/HP1 alpha/CBX5 rabbit polyclonal antibody antibody (STJ22922) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of HeLa cells using HP1 alpha/HP1 alpha/CBX5 antibody (STJ22922). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug HP1 alpha/HP1 alpha/CBX5 antibody (STJ22922). Western blot was performed from the immunoprecipitate using HP1 alpha/HP1 alpha/CBX5 antibody (STJ22922) at a dilition of 1:1000.

Anti-CBX5 antibody (1-191) (STJ22922)

SKU:
STJ22922

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CBX5 (1-191) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:50-1:200
ChIP 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CBX5
Gene ID: 23468
Uniprot ID: CBX5_HUMAN
Immunogen Region: 1-191
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human HP1 alpha/HP1 alpha/CBX5 (NP_001120794.1).
Immunogen Sequence: MGKKTKRTADSSSSEDEEEY VVEKVLDRRVVKGQVEYLLK WKGFSEEHNTWEPEKNLDCP ELISEFMKKYKKMKEGENNK PREKSESNKRKSNFSNSADD IKSKKKREQSNDIARGFERG LEPEKIIGATDSCGDLMFLM KWKDTDEADLVLAKEANVKC PQIVIAFYEERLTWHAYPED AENKEKETAKS
Post Translational Modifications Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis. Ubiquitinated.
Function Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Protein Name Chromobox Protein Homolog 5
Antigen P25
Heterochromatin Protein 1 Homolog Alpha
Hp1 Alpha
Database Links Reactome: R-HSA-4551638
Reactome: R-HSA-8953750
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Chromosome
Centromere
Colocalizes With Hnrnpu In The Nucleus
Component Of Centromeric And Pericentromeric Heterochromatin
Associates With Chromosomes During Mitosis
Associates Specifically With Chromatin During Metaphase And Anaphase
Localizes To Sites Of Dna Damage
Alternative Antibody Names Anti-Chromobox Protein Homolog 5 antibody
Anti-Antigen P25 antibody
Anti-Heterochromatin Protein 1 Homolog Alpha antibody
Anti-Hp1 Alpha antibody
Anti-CBX5 antibody
Anti-HP1A antibody

Information sourced from Uniprot.org

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