• Western blot analysis of extracts of MCF7 cells, using CD82 antibody (STJ28271) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded human colon using CD82 antibody (STJ28271) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human spleen using CD82 antibody (STJ28271) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse brain using CD82 antibody (STJ28271) at dilution of 1:100 (40x lens).

Anti-CD82 antibody (150-250) (STJ28271)

SKU:
STJ28271

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD82 (150-250) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD82
Gene ID: 3732
Uniprot ID: CD82_HUMAN
Immunogen Region: 150-250
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human CD82 (NP_002222.1).
Immunogen Sequence: CGWVSFYNWTDNAELMNRPE VTYPCSCEVKGEEDNSLSVR KGFCEAPGNRTQSGNHPEDW PVYQEGCMEKVQAWLQENLG IILGVGVGVAIIELLGMVLS I
Tissue Specificity Lymphoid specific.
Function Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
Protein Name Cd82 Antigen
C33 Antigen
Ia4
Inducible Membrane Protein R2
Metastasis Suppressor Kangai-1
Suppressor Of Tumorigenicity 6 Protein
Tetraspanin-27
Tspan-27
Cd Antigen Cd82
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Cd82 Antigen antibody
Anti-C33 Antigen antibody
Anti-Ia4 antibody
Anti-Inducible Membrane Protein R2 antibody
Anti-Metastasis Suppressor Kangai-1 antibody
Anti-Suppressor Of Tumorigenicity 6 Protein antibody
Anti-Tetraspanin-27 antibody
Anti-Tspan-27 antibody
Anti-Cd Antigen Cd82 antibody
Anti-CD82 antibody
Anti-KAI1 antibody
Anti-SAR2 antibody
Anti-ST6 antibody
Anti-TSPAN27 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance