• Western blot analysis of extracts of mouse spinal cord, using CHRM5 antibody (STJ27320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CHRM5 antibody (275-370) (STJ27320)

SKU:
STJ27320

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHRM5 (275-370) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHRM5
Gene ID: 1133
Uniprot ID: ACM5_HUMAN
Immunogen Region: 275-370
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 275-370 of human CHRM5 (NP_036257.1).
Immunogen Sequence: RRSTSTTGKPSQATGPSANW AKAEQLTTCSSYPSSEDEDK PATDPVLQVVYKSQGKESPG EEFSAEETEETFVKAETEKS DYDTPNYLLSPAAAHR
Function The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Protein Name Muscarinic Acetylcholine Receptor M5
Database Links Reactome: R-HSA-390648
Reactome: R-HSA-416476
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Postsynaptic Cell Membrane
Alternative Antibody Names Anti-Muscarinic Acetylcholine Receptor M5 antibody
Anti-CHRM5 antibody

Information sourced from Uniprot.org

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