• Endogenous CX43 detected at 1:1000 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Immunohistochemistry of mouse spleen using CX43 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CX43 antibody at 1:500:DAB detection
  • Immunohistochemistry of human skin using CX43 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CX43 antibody at 1:500:DAB detection
  • Immunohistochemistry of mouse lung using anti-CX43 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CX43 antibody at 1:500:DAB detection
  • Immunohistochemistry of mouse pancreas using anti CX43 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CX43 antibody at 1:500:DAB detection
  • Immunohistochemistry of mouse brain using anti-CX43 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CX43 antibody at 1:500:DAB detection
  • Immunostaining of COS7 cells with CX43 antibody at 1:250 dilution and Dylight 550
  • Immunostaining of primary RPE cells with CX43 antibody at 1:100 dilution
  • Immunohistochemistry of human skin using CX43 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CX43 antibody at 1:500/DAB detection

Anti-GJA1 antibody (230aa C-Term) (STJ140032)

SKU:
STJ140032

Current Stock:
Host: Goat
Applications: WB/IHC-F/IHC-P/IF
Reactivity: Human/Rat/Mouse/Monkey/Canine
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Gap junction protein, alpha 1, 43kDa (230aa C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 4 mg/ml
Dilution Range: WB 1:500-1:2000
IF 1:50-1:250
IHC-P 1:250-1:800
IHC-F 1:250-1:800
Martins-Marques T Costa MC Catarino S et al. EMBO Rep 2022 May PMID: 35593040
Lissoni A Hulpiau P Martins-Marques T et al. Cardiovasc Res. 2021 Jan PMID: 31841141
Storage Instruction: Store at-20 C for long-term storage. Store at 2-8 C for up to one month.
Gene Symbol: GJA1
Gene ID: 2697
Uniprot ID: CXA1_HUMAN
Immunogen Region: 230aa C-Term
Accession Number: ENSG00000152661
Specificity: Detects endogenous levels of total connexin 43.
Immunogen: Purified recombinant peptide derived from within residues 230 aa to the C-terminus of rat CX43 produced in E. coli.
Immunogen Sequence: VKDRVKGRSDPYHATTGPLS PSKDCGSPKYAYFNGCSSPT APLSPMSPPGYKLVTGDRNN SSCRNYNKQASEQNWANYSA EQNRMGQAGSTISNSHAQPF DFPDDNQNAKKVAAGHELQP LAIVDQRPSSRASSRASSRP RPDDLEI
Tissue Specificity Expressed in the heart and fetal cochlea.
Post Translational Modifications Phosphorylated at Ser-368 by PRKCG.phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity. Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation. Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2. S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication. Acetylated in the developing cortex.leading to delocalization from the cell membrane.
Function Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles.
Protein Name Gap Junction Alpha-1 Protein
Connexin-43
Cx43
Gap Junction 43 Kda Heart Protein
Database Links Reactome: R-HSA-190704
Reactome: R-HSA-190827
Reactome: R-HSA-190840
Reactome: R-HSA-190861
Reactome: R-HSA-190873
Reactome: R-HSA-191650
Reactome: R-HSA-196025
Reactome: R-HSA-9013406
Reactome: R-HSA-9013409
Reactome: R-HSA-9705677
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Junction
Gap Junction
Endoplasmic Reticulum
Localizes At The Intercalated Disk (Icd) In Cardiomyocytes And The Proper Localization At Icd Is Dependent On Tmem65
Alternative Antibody Names Anti-Gap Junction Alpha-1 Protein antibody
Anti-Connexin-43 antibody
Anti-Cx43 antibody
Anti-Gap Junction 43 Kda Heart Protein antibody
Anti-GJA1 antibody
Anti-GJAL antibody

Information sourced from Uniprot.org

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