• Endogenous ERP57-endoplasmic reticulum lumen marker detected at 1:500 dilution; lysates at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Immunofluorescence – anti-ERP57 antibody in primary RPE cells at 1:100 dilution; cells were fixed with 4% of PFA
  • Immunofluorescence – anti-ERP57 antibody in primary RPE cells at 1:100 dilution; cells were fixed with 4% of PFA
  • Immunofluorescence – anti-ERP57 antibody in primary RPE cells at 1/100 dilution; cells were fixed with 4% of PFA
  • Immunohistochemistry of human appendix using anti-ERp57 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-ERp57 antibody at 1:750/DAB detection
  • Immunohistochemistry of mouse retina using anti-ERp57 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-ERp57 antibody at 1:750/DAB detection;
  • Immunohistochemistry of human gallbladder using anti-ERp57 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-ERp57 antibody at 1:750/DAB detection
  • Immunohistochemistry of mouse kidney using anti-ERp57 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-ERp57 antibody at 1:750/DAB detection
  • Immunohistochemistry of human pancreas using anti-ERp57 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-ERp57 antibody at 1:750/DAB detection
  • Endogenous ERP57-endoplasmic reticulum lumen marker detected at 1/1,000 dilution, CANX- STJ140016 at 1/5,000 and GAPDH- STJ140037 at 1/2, 500; lysates at 50 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution

Anti-PDIA3 antibody (300aa C-Term) (STJ140036)

SKU:
STJ140036

Current Stock:
Host: Goat
Applications: WB/IHC-F/IHC-P/IF
Reactivity: Human/Rat/Mouse/Monkey/Canine
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PDIA3 protein disulfide isomerase family A, member 3 (300aa C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3 mg/ml
Dilution Range: WB 1:500-1:2000
IF 1:50-1:500
IHC-P 1:200-1:1000
IHC-F 1:200-1:1000
Storage Instruction: Store at-20 C for long-term storage. Store at 2-8 C for up to one month.
Gene Symbol: PDIA3
Gene ID: 2923
Uniprot ID: PDIA3_HUMAN
Immunogen Region: 300aa C-Term
Accession Number: ENSG00000167004
Specificity: Detects a band of 60 kDa by Western blot in the following canine, human, monkey, mouse, rat whole cell lysates.
Immunogen: Recombinant peptide derived from within residues 300 aa to the C-terminus of human ERp57 produced in E. coli.
Immunogen Sequence: STAGEIPVVAIRTAKGEKFV MQEEFSRDGKALERFLQDYF DGNLKRYLKSEPIPESNDGP VKVVVAENFDEIVNNENKDV LIEFYAPWCGHCKNLEPKYK ELGEKLSKDPNIVIAKMDAT ANDVPSPYEVRGFPTIYFSP ANKKLNPKKYEGGRELSDFI SYLQREATNPPVIQEEKPKK KKKAQEDL
Tissue Specificity Detected in the flagellum and head region of spermatozoa (at protein level). Expressed in liver, stomach and colon (at protein level). Expressed in gastric parietal cells and chief cells (at protein level).
Post Translational Modifications Within the major histocompatibility complex class I (MHC I) peptide loading complex forms reversible disulfide-linked heterodimers with TAPBP as part of its protein folding chaperone activity. This is essential to assist the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Phosphorylated.
Function Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone. Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity.
Protein Name Protein Disulfide-Isomerase A3
58 Kda Glucose-Regulated Protein
58 Kda Microsomal Protein
P58
Disulfide Isomerase Er-60
Endoplasmic Reticulum Resident Protein 57
Er Protein 57
Erp57
Endoplasmic Reticulum Resident Protein 60
Er Protein 60
Erp60
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-901042
Reactome: R-HSA-983170
Cellular Localisation Endoplasmic Reticulum
Endoplasmic Reticulum Lumen
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Protein Disulfide-Isomerase A3 antibody
Anti-58 Kda Glucose-Regulated Protein antibody
Anti-58 Kda Microsomal Protein antibody
Anti-P58 antibody
Anti-Disulfide Isomerase Er-60 antibody
Anti-Endoplasmic Reticulum Resident Protein 57 antibody
Anti-Er Protein 57 antibody
Anti-Erp57 antibody
Anti-Endoplasmic Reticulum Resident Protein 60 antibody
Anti-Er Protein 60 antibody
Anti-Erp60 antibody
Anti-PDIA3 antibody
Anti-ERP57 antibody
Anti-ERP60 antibody
Anti-GRP58 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance