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Anti-GAPDH antibody

[STJ96931] Download PDF Print Data Sheet

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Product name Anti-GAPDH antibody
Short Description Mouse Monoclonal to GAPDH.
Description GAPDH is a protein encoded by the GAPDH gene which is approximately 36 kDa. GAPDH is localised to the cytoplasm and nucleus. It is involved in glucose metabolism, respiratory electron transport, carbon metabolism and HIF-1 signalling pathway. It is a moonlighting protein based on its ability to perform mechanistically distinct functions. It catalyses an important energy-yielding step in carbohydrate metabolism and also has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions. GAPDH is expressed in the blood, eyes, intestine, kidney and liver. Mutations in the GAPDH gene may result in FMR1-related disorders. STJ96931 was developed from clone 2B8 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody detects endogenous GAPDH protein.
Applications IHC-p, WB
Dilution range WB 1:5000
IHC 1:200
Specificity The antibody detects endogenous GAPDH protein.
Protein Name Glyceraldehyde-3-phosphate dehydrogenase
GAPDH
Peptidyl-cysteine S-nitrosylase GAPDH
Immunogen Synthetic Peptide
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Mouse
Clonality Monoclonal
Clone ID 2B8
Reactivity Cow, Dog, Hamster, Human, Insect, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Yeast
Conjugation Unconjugated
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Isotype IgG1
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 2597
Gene Symbol GAPDH
Database Links HGNC:4141
OMIM:138400
Alternative Names GAPDH antibody
GAPD antibody
CDABP0047 antibody
OK/SW-cl.12 antibody
Glyceraldehyde-3-phosphate dehydrogenase antibody
GAPDH antibody
Peptidyl-cysteine S-nitrosylase GAPDH antibody
Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Post-translational Modifications S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus (By similarity). S-nitrosylation of Cys-247 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity. ; ISGylated. ; Sulfhydration at Cys-152 increases catalytic activity. ; Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at Met-46 may play a pivotal role in the formation of these insoluble structures. This modification has been detected in vitro following treatment with free radical donor (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide. It has been proposed to destabilize nearby residues, increasing the likelihood of secondary oxidative damages, including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and aggregation.
Cellular Localization Cytoplasm, cytosol Nucleus Cytoplasm, perinuclear region Membrane Cytoplasm, cytoskeleton Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
Sequence and Domain Family The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

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