• Western blot analysis of extracts of various cell lines, using GDI1 antibody (STJ27415) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-GDI1 antibody (188-447) (STJ27415)

SKU:
STJ27415

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GDI1 (188-447) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GDI1
Gene ID: 2664
Uniprot ID: GDIA_HUMAN
Immunogen Region: 188-447
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 188-447 of human GDI1 (NP_001484.1).
Immunogen Sequence: ALALYRTDDYLDQPCLETVN RIKLYSESLARYGKSPYLYP LYGLGELPQGFARLSAIYGG TYMLNKPVDDIIMENGKVVG VKSEGEVARCKQLICDPSYI PDRVRKAGQVIRIICILSHP IKNTNDANSCQIIIPQNQVN RKSDIYVCMISYAHNVAAQG KYIAIASTTVETTDPEKEVE PALELLEPIDQKFVAISDLY EPIDDGCESQVFCSCSYDAT THFETTCNDIKDIYKRMAG
Tissue Specificity Brain.predominant in neural and sensory tissues.
Function Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Promotes the dissociation of GDP-bound Rab proteins from the membrane and inhibits their activation. Promotes the dissociation of RAB1A, RAB3A, RAB5A and RAB10 from membranes.
Protein Name Rab Gdp Dissociation Inhibitor Alpha
Rab Gdi Alpha
Guanosine Diphosphate Dissociation Inhibitor 1
Gdi-1
Oligophrenin-2
Protein Xap-4
Database Links Reactome: R-HSA-8876198
Cellular Localisation Cytoplasm
Golgi Apparatus
Trans-Golgi Network
Alternative Antibody Names Anti-Rab Gdp Dissociation Inhibitor Alpha antibody
Anti-Rab Gdi Alpha antibody
Anti-Guanosine Diphosphate Dissociation Inhibitor 1 antibody
Anti-Gdi-1 antibody
Anti-Oligophrenin-2 antibody
Anti-Protein Xap-4 antibody
Anti-GDI1 antibody
Anti-GDIL antibody
Anti-OPHN2 antibody
Anti-RABGDIA antibody
Anti-XAP4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance