• Western blot analysis of extracts of various cell lines, using GDI2 antibody (STJ111343) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of NIH/3T3 cells using GDI2 antibody (STJ111343) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts from normal (control) and GDI2 knockout (KO) 293T cells, using GDI2 antibody (STJ111343) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts from normal (control) and GDI2 knockout (KO) 293T cells, using GDI2 antibody (STJ111343) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of NIH/3T3 cells using GDI2 antibody (STJ111343) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using GDI2 antibody (STJ111343) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GDI2 antibody (296-445) (STJ111343)

SKU:
STJ111343

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GDI2 (296-445) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:3000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GDI2
Gene ID: 2665
Uniprot ID: GDIB_HUMAN
Immunogen Region: 296-445
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 296-445 of human GDI2 (NP_001485.2).
Immunogen Sequence: QVIRVICILSHPIKNTNDAN SCQIIIPQNQVNRKSDIYVC MISFAHNVAAQGKYIAIVST TVETKEPEKEIRPALELLEP IEQKFVSISDLLVPKDLGTE SQIFISRTYDATTHFETTCD DIKNIYKRMTGSEFDFEEMK RKKNDIYGED
Tissue Specificity Ubiquitous.
Function GDP-dissociation inhibitor preventing the GDP to GTP exchange of most Rab proteins. By keeping these small GTPases in their inactive GDP-bound form regulates intracellular membrane trafficking. Negatively regulates protein transport to the cilium and ciliogenesis through the inhibition of RAB8A.
Protein Name Rab Gdp Dissociation Inhibitor Beta
Rab Gdi Beta
Guanosine Diphosphate Dissociation Inhibitor 2
Gdi-2
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-8876198
Cellular Localisation Cytoplasm
Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Rab Gdp Dissociation Inhibitor Beta antibody
Anti-Rab Gdi Beta antibody
Anti-Guanosine Diphosphate Dissociation Inhibitor 2 antibody
Anti-Gdi-2 antibody
Anti-GDI2 antibody
Anti-RABGDIB antibody

Information sourced from Uniprot.org

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