• Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GLI-3 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of HepG2 cells, using GLI-3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-GLI3 antibody (11-60 aa) (STJ93279)

SKU:
STJ93279

Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Transcriptional activator GLI3 (11-60 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GLI3
Gene ID: 2737
Uniprot ID: GLI3_HUMAN
Immunogen Region: 11-60 aa
Specificity: GLI-3 Polyclonal Antibody detects endogenous levels of GLI-3 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human GLI-3 at the amino acid range 11-60
Post Translational Modifications Phosphorylated on multiple sites by protein kinase A (PKA) and phosphorylation by PKA primes further phosphorylation by CK1 and GSK3. Phosphorylated by DYRK2 (in vitro). Phosphorylation is essential for its proteolytic processing. Transcriptional repressor GLI3R, a C-terminally truncated form, is generated from the full-length GLI3 protein (GLI3FL/GLI3-190) through proteolytic processing. This process requires PKA-primed phosphorylation of GLI3, ubiquitination of GLI3 and the presence of BTRC. GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. GLI3R formation leads to its dissociation from SUFU, allowing it to translocate into the nucleus, and repress Hh target genes. When Hh signaling is initiated, SUFU dissociates from GLI3FL and this has two consequences. First, GLI3R production is halted. Second, free GLI3FL translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A). Phosphorylated in vitro by ULK3.
Function Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
Protein Name Transcriptional Activator Gli3
Gli3 Form Of 190 Kda
Gli3-190
Gli3 Full-Length Protein
Gli3fl Cleaved Into - Transcriptional Repressor Gli3r
Gli3 C-Terminally Truncated Form
Gli3 Form Of 83 Kda
Gli3-83
Database Links Reactome: R-HSA-5610785
Reactome: R-HSA-5610787
Reactome: R-HSA-5632684
Reactome: R-HSA-5635851
Reactome: R-HSA-8940973
Cellular Localisation Nucleus
Cytoplasm
Cell Projection
Cilium
Gli3fl Is Localized Predominantly In The Cytoplasm While Gli3r Resides Mainly In The Nucleus
Ciliary Accumulation Requires The Presence Of Kif7 And Smo
Translocation To The Nucleus Is Promoted By Interaction With Zic1
Alternative Antibody Names Anti-Transcriptional Activator Gli3 antibody
Anti-Gli3 Form Of 190 Kda antibody
Anti-Gli3-190 antibody
Anti-Gli3 Full-Length Protein antibody
Anti-Gli3fl Cleaved Into - Transcriptional Repressor Gli3r antibody
Anti-Gli3 C-Terminally Truncated Form antibody
Anti-Gli3 Form Of 83 Kda antibody
Anti-Gli3-83 antibody
Anti-GLI3 antibody

Information sourced from Uniprot.org

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