• Immunoprecipitation analysis of 200 Mu g extracts of HT-29 cells, using 3 Mu g [KD Validated] HDAC1 Rabbit polyclonal antibody (STJ23928). Western blot was performed from the immunoprecipitate using HDAC1 antibody (STJ23928) at a dilution of 1:1000.
  • Confocal immunofluorescence analysis of L929 cells using [KD Validated] HDAC1 Rabbit polyclonal antibody (STJ23928) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A549 cells using [KD Validated] HDAC1 Rabbit polyclonal antibody (STJ23928). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of HDAC1 in paraffin-embedded human colon using [KD Validated] HDAC1 Rabbit polyclonal antibody (STJ23928) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HDAC1 in paraffin-embedded human lung cancer using [KD Validated] HDAC1 Rabbit polyclonal antibody (STJ23928) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HDAC1 in paraffin-embedded rat spleen using [KD Validated] HDAC1 Rabbit polyclonal antibody (STJ23928) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and HDAC1 knockdown (KD) 293T cells, using [KD Validated] HDAC1 Rabbit polyclonal antibody (STJ23928) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates using [KD Validated] HDAC1 Rabbit polyclonal antibody (STJ23928) at 1:2000 dilution incubated at room temperature for 1. 5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic KitExposure time: 20 s.

Anti-HDAC1 antibody (393-482) (STJ23928)

SKU:
STJ23928

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HDAC1 (393-482) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HDAC1
Gene ID: 3065
Uniprot ID: HDAC1_HUMAN
Immunogen Region: 393-482
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2).
Immunogen Sequence: SGDEDEDDPDKRISICSSDK RIACEEEFSDSEEEGEGGRK NSSNFKKAKRVKTEDEKEKD PEEKKEVTEEEKTKEEKPEA KGVKEEVKLA
Tissue Specificity Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
Post Translational Modifications Sumoylated on Lys-444 and Lys-476.which promotes enzymatic activity. Desumoylated by SENP1. Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes. Phosphorylated by CDK5. Ubiquitinated by CHFR, leading to its degradation by the proteasome. Ubiquitinated by KCTD11, leading to proteasomal degradation.
Function Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Also functions as deacetylase for non-histone targets, such as NR1D2, RELA, SP1, SP3 and TSHZ3. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation. In addition to protein deacetylase activity, also has protein-lysine deacylase activity: acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones.
Protein Name Histone Deacetylase 1
Hd1
Protein Deacetylase Hdac1
Protein Decrotonylase Hdac1
Database Links Reactome: R-HSA-1362277
Reactome: R-HSA-1362300
Reactome: R-HSA-1538133
Reactome: R-HSA-193670
Reactome: R-HSA-201722
Reactome: R-HSA-2122947
Reactome: R-HSA-2173795
Reactome: R-HSA-2173796
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-3214815
Reactome: R-HSA-350054
Reactome: R-HSA-3769402
Reactome: R-HSA-427389
Reactome: R-HSA-427413
Reactome: R-HSA-4551638
Reactome: R-HSA-4641265
Reactome: R-HSA-6804758
Reactome: R-HSA-69205
Reactome: R-HSA-73762
Reactome: R-HSA-8936459
Reactome: R-HSA-8943724
Reactome: R-HSA-9018519
Reactome: R-HSA-9022538
Reactome: R-HSA-9022692
Reactome: R-HSA-9022699
Reactome: R-HSA-9022702
Reactome: R-HSA-9615017
Reactome: R-HSA-9679191
Reactome: R-HSA-9701898
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Histone Deacetylase 1 antibody
Anti-Hd1 antibody
Anti-Protein Deacetylase Hdac1 antibody
Anti-Protein Decrotonylase Hdac1 antibody
Anti-HDAC1 antibody
Anti-RPD3L1 antibody

Information sourced from Uniprot.org

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