• Western blot analysis of the lysates from HUVECcells using MEKKK 1 antibody.
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from HepG2 cells, using MEKKK 1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using HPK1 Polyclonal Antibody diluted at 1：1000

Anti-MAP4K1 antibody (371-420 aa) (STJ93590)

SKU:
STJ93590

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mitogen-activated protein kinase kinase kinase kinase 1 (371-420 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP4K1
Gene ID: 11184
Uniprot ID: M4K1_HUMAN
Immunogen Region: 371-420 aa
Specificity: HPK1 Polyclonal Antibody detects endogenous levels of HPK1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MEKKK 1 at the amino acid range 371-420
Post Translational Modifications Autophosphorylates: phosphorylation promotes ubiquitination by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex, leading to its degradation by the proteasome. Tyrosine-phosphorylated after activation of hemopoietic cells. Ubiquitinated by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex following autophosphorylation, leading to its degradation by the proteasome.
Function Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate. Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production.
Protein Name Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1
Hematopoietic Progenitor Kinase
Mapk/Erk Kinase Kinase Kinase 1
Mek Kinase Kinase 1
Mekkk 1
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 antibody
Anti-Hematopoietic Progenitor Kinase antibody
Anti-Mapk/Erk Kinase Kinase Kinase 1 antibody
Anti-Mek Kinase Kinase 1 antibody
Anti-Mekkk 1 antibody
Anti-MAP4K1 antibody
Anti-HPK1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance