• Western blot analysis of extracts of various cell lines, using INPP5A antibody (STJ26233) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-INPP5A antibody (10-200) (STJ26233)

SKU:
STJ26233

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-INPP5A (10-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: INPP5A
Gene ID: 3632
Uniprot ID: I5P1_HUMAN
Immunogen Region: 10-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 10-200 of human INPP5A (NP_005530.3).
Immunogen Sequence: TAVLLVTANVGSLFDDPENL QKNWLREFYQVVHTHKPHFM ALHCQEFGGKNYEASMSHVD KFVKELLSSDAMKEYNRARV YLDENYKSQEHFTALGSFYF LHESLKNIYQFDFKAKKYRK VAGKEIYSDTLESTPMLEKE KFPQDYFPECKWSRKGFIRT RWCIADCAFDLVNIHLFHDA SNLVAWETSPS
Tissue Specificity Predominantly expressed in heart, brain, and skeletal muscle. In brain.high level in Purkinje cells.
Post Translational Modifications Isoprenylation at Cys-409 is required for localization at the membrane.
Function Phosphatase that specifically hydrolyzes the 5-phosphate of inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate, and inositol 1,3,4,5-tetrasphosphate to inositol 1,3,4-trisphosphate. Plays a crucial role in the survival of cerebellar Purkinje cells.
Protein Name Inositol Polyphosphate-5-Phosphatase A
43 Kda Inositol Polyphosphate 5-Phosphatase
Type I Inositol 1 -4 -5-Trisphosphate 5-Phosphatase
5ptase
Database Links Reactome: R-HSA-1855183
Cellular Localisation Cell Membrane
Lipid-Anchor
Cell Projection
Dendrite
Alternative Antibody Names Anti-Inositol Polyphosphate-5-Phosphatase A antibody
Anti-43 Kda Inositol Polyphosphate 5-Phosphatase antibody
Anti-Type I Inositol 1 -4 -5-Trisphosphate 5-Phosphatase antibody
Anti-5ptase antibody
Anti-INPP5A antibody
Anti-5PTASE antibody

Information sourced from Uniprot.org

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