• Western blot analysis of extracts of various cell lines, using INPP5B antibody (STJ116222) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
  • Immunofluorescence analysis of C6 cells using INPP5B antibody (STJ116222) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using INPP5B antibody (STJ116222) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-INPP5B antibody (1-260) (STJ116222)

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STJ116222

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-INPP5B (1-260) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: INPP5B
Gene ID: 3633
Uniprot ID: I5P2_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human INPP5B (NP_005531.2).
Immunogen Sequence: MDQSVAIQETLAEGEYCVIA VQGVLCEGDSRQSRLLGLVR YRLEHGGQEHALFLYTHRRM AITGDDVSLDQIVPVSRDFT LEEVSPDGELYILGSDVTVQ LDTAELSLVFQLPFGSQTRM FLHEVARACPGFDSATRDPE FLWLSRYRCAELELEMPTPR GCNSALVTWPGYATIGGGGS NFDGLRPNGKGVPMDQSSRG QDKPESLQPRQNKSKSEITD MVRSSTITVSDKAHILSMQ
Tissue Specificity Platelets.
Post Translational Modifications Isoprenylation at Cys-990 may be required for localization at the membrane. May be proteolytically cleaved after Lys-320 as inferred from N-terminal protein sequence of the 75 kda form.
Function Hydrolyzes phosphatidylinositol 4,5-bisphosphate (PtIns(4,5)P2) and the signaling molecule phosphatidylinositol 1,4,5-trisphosphate (PtIns(1,4,5)P3), and thereby modulates cellular signaling events.
Protein Name Type Ii Inositol 1 -4 -5-Trisphosphate 5-Phosphatase
75 Kda Inositol Polyphosphate-5-Phosphatase
Phosphoinositide 5-Phosphatase
5ptase
Database Links Reactome: R-HSA-1855183
Reactome: R-HSA-1855204
Cellular Localisation Cytoplasm
Cytosol
Endoplasmic Reticulum-Golgi Intermediate Compartment
Early Endosome Membrane
Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasmic Vesicle
Phagosome Membrane
Golgi Apparatus
Alternative Antibody Names Anti-Type Ii Inositol 1 -4 -5-Trisphosphate 5-Phosphatase antibody
Anti-75 Kda Inositol Polyphosphate-5-Phosphatase antibody
Anti-Phosphoinositide 5-Phosphatase antibody
Anti-5ptase antibody
Anti-INPP5B antibody
Anti-OCRL2 antibody

Information sourced from Uniprot.org

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