• Western blot analysis of lysates from 1) HepG2, 2) 293T, 3) Mouse Brain Tissue, 4) Rat Brain Tissue cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody (cat: (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
  • 1) Input: Mouse Brain Tissue Lysate , 2) IP product: IP dilute 1:200
  • Western blot analysis of 1) HepG2, 2) 293T, 3) Mouse Brain Tissue, 4) Rat Brain Tissue, diluted at 1:5000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Immunofluorescence analysis of Human-lung-cancer tissue. 1, Lamin B1 monoclonal antibody (7C11) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Lamin B1 monoclonal antibody (7C11) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of Hela cell. 1, AMPK Alpha 1/2 (phospho Thr183/172) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-LMNB1 antibody [7C11] (STJ96938)

SKU:
STJ96938

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF/IP
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Lamin-B1 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: 7C11
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 1:2000-5000
IP 1:200
IF 1:200
IHC 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LMNB1
Gene ID: 4001
Uniprot ID: LMNB1_HUMAN
Specificity: The antibody detects endogenous Lamin B1 protein.
Immunogen: Recombinant Protein of Lamin-B1
Function Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Protein Name Lamin-B1
Database Links Reactome: R-HSA-1221632
Reactome: R-HSA-2559584
Reactome: R-HSA-2980766
Reactome: R-HSA-2995383
Reactome: R-HSA-352238
Reactome: R-HSA-4419969
Reactome: R-HSA-8862803
Reactome: R-HSA-8950505
Reactome: R-HSA-9013405
Reactome: R-HSA-9035034
Cellular Localisation Nucleus Lamina
Alternative Antibody Names Anti-Lamin-B1 antibody
Anti-LMNB1 antibody
Anti-LMN2 antibody
Anti-LMNB antibody

Information sourced from Uniprot.org

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