• Anti-Luciferase antibody at 1:500 dilution; 293HEK transduced with lentivirus expressing luciferase; lysates at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-Firefly Luciferase antibody (340aa C-Term) (STJ140083)

SKU:
STJ140083

Current Stock:
Host: Goat
Applications: WB
Reactivity: Transfected cells
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Firefly Luciferase (340aa C-Term) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3 mg/ml
Dilution Range: WB 1:500-1:2000
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Immunogen Region: 340aa C-Term
Specificity: In 293HEK cells transfected with cds plasmid detects a band of 62 kDa by Western blot.
Immunogen: Purified recombinant peptide derived from within residues 340 aa to the C-terminus of Luciferase produced in E. coli.
Background Goat polyclonal antibody to Firefly luciferase. Luciferase is a widely used reporter protein to study gene expression. Luciferase catalyses a bioluminescent reaction which requires luciferin as a substrate, ATP and Mg2+. A cell extract containing luciferase mixed luciferin in the presence of ATP and Mg2+, results in a flash of light that decays rapidly and can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Information sourced from Uniprot.org

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