• Western blot analysis of extracts of various cell lines, using MMP2 antibody (STJ28003) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using MMP2 antibody (STJ28003) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry of paraffin-embedded human appendix using MMP2 antibody (STJ28003) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using MMP2 antibody (STJ28003) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using MMP2 antibody (STJ28003) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MMP2 antibody (451-660) (STJ28003)

SKU:
STJ28003

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MMP2 (451-660) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MMP2
Gene ID: 4313
Uniprot ID: MMP2_HUMAN
Immunogen Region: 451-660
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 451-660 of human MMP2 (NP_004521.1).
Immunogen Sequence: IDLGTGPTPTLGPVTPEICK QDIVFDGIAQIRGEIFFFKD RFIWRTVTPRDKPMGPLLVA TFWPELPEKIDAVYEAPQEE KAVFFAGNEYWIYSASTLER GYPKPLTSLGLPPDVQRVDA AFNWSKNKKTYIFAGDKFWR YNEVKKKMDPGFPKLIADAW NAIPDNLDAVVDLQGGGHSY FFKGAYYLKLENQSLKSVKF GSIKSDWLGC
Tissue Specificity Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
Post Translational Modifications Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro. The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
Function Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
Protein Name 72 Kda Type Iv Collagenase
72 Kda Gelatinase
Gelatinase A
Matrix Metalloproteinase-2
Mmp-2
Tbe-1 Cleaved Into - Pex
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Reactome: R-HSA-381426
Reactome: R-HSA-3928665
Reactome: R-HSA-6785807
Reactome: R-HSA-9009391
Cellular Localisation Isoform 1: Secreted
Extracellular Space
Extracellular Matrix
Membrane
Nucleus
Colocalizes With Integrin Alphav/Beta3 At The Membrane Surface In Angiogenic Blood Vessels And Melanomas
Found In Mitochondria
Along Microfibrils
And In Nuclei Of Cardiomyocytes
Isoform 2: Cytoplasm
Mitochondrion
Alternative Antibody Names Anti-72 Kda Type Iv Collagenase antibody
Anti-72 Kda Gelatinase antibody
Anti-Gelatinase A antibody
Anti-Matrix Metalloproteinase-2 antibody
Anti-Mmp-2 antibody
Anti-Tbe-1 Cleaved Into - Pex antibody
Anti-MMP2 antibody
Anti-CLG4A antibody

Information sourced from Uniprot.org

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