• Western blot analysis of extracts of mouse kidney, using NOTCH4 antibody (STJ110601) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of L929 cells using NOTCH4 rabbit polyclonal antibody (STJ110601) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-NOTCH4 antibody (1824-2003) (STJ110601)

SKU:
STJ110601

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NOTCH4 (1824-2003) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOTCH4
Gene ID: 4855
Uniprot ID: NOTC4_HUMAN
Immunogen Region: 1824-2003
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1824-2003 of human NOTCH4 (NP_004548.3).
Immunogen Sequence: PPEARHKATPGREAGPFPRA RTVSVSVPPHGGGALPRCRT LSAGAGPRGGGACLQARTWS VDLAARGGGAYSHCRSLSGV GAGGGPTPRGRRFSAGMRGP RPNPAIMRGRYGVAAGRGGR VSTDDWPCDWVALGACGSAS NIPIPPPCLTPSPERGSPQL DCGPPALQEMPINQGGEGKK
Tissue Specificity Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.
Post Translational Modifications Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated.
Function Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.
Protein Name Neurogenic Locus Notch Homolog Protein 4
Notch 4
Hnotch4 Cleaved Into - Notch 4 Extracellular Truncation - Notch 4 Intracellular Domain
Database Links Reactome: R-HSA-1912399
Reactome: R-HSA-1912408
Reactome: R-HSA-1912420
Reactome: R-HSA-350054
Reactome: R-HSA-5083630
Reactome: R-HSA-9013695
Reactome: R-HSA-9013700
Reactome: R-HSA-9604323
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Notch 4 Intracellular Domain: Nucleus
Following Proteolytical Processing Nicd Is Translocated To The Nucleus
Alternative Antibody Names Anti-Neurogenic Locus Notch Homolog Protein 4 antibody
Anti-Notch 4 antibody
Anti-Hnotch4 Cleaved Into - Notch 4 Extracellular Truncation - Notch 4 Intracellular Domain antibody
Anti-NOTCH4 antibody
Anti-INT3 antibody

Information sourced from Uniprot.org

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