• Immunohistochemistry analysis of CD73/NT5E in paraffin-embedded human tonsil using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CD73/NT5E in paraffin-embedded human liver cancer using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and CD73/NT5E knockout (KO) HCT116 cells, using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from Rat eye, using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NT5E antibody (27-574) (STJ24827)

SKU:
STJ24827

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NT5E/CD73 (27-574) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NT5E
Gene ID: 4907
Uniprot ID: 5NTD_HUMAN
Immunogen Region: 27-574
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-574 of human CD73/NT5E (NP_002517.1).
Immunogen Sequence: WELTILHTNDVHSRLEQTSE DSSKCVNASRCMGGVARLFT KVQQIRRAEPNVLLLDAGDQ YQGTIWFTVYKGAEVAHFMN ALRYDAMALGNHEFDNGVEG LIEPLLKEAKFPILSANIKA KGPLASQISGLYLPYKVLPV GDEVVGIVGYTSKETPFLSN PGTNLVFEDEITALQPEVDK LKTLNVNKIIALGHSGFEMD KLIAQKVRGVDVVVGGHSNT FLYTGNPPSKEVPAGKYPF
Function Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN.
Protein Name 5'-Nucleotidase
5'-Nt
5'-Deoxynucleotidase
Ecto-5'-Nucleotidase
Imp-Specific 5'-Nucleotidase
Thymidylate 5'-Phosphatase
Cd Antigen Cd73
Database Links Reactome: R-HSA-196807
Reactome: R-HSA-73621
Reactome: R-HSA-74259
Reactome: R-HSA-9660826
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-5'-Nucleotidase antibody
Anti-5'-Nt antibody
Anti-5'-Deoxynucleotidase antibody
Anti-Ecto-5'-Nucleotidase antibody
Anti-Imp-Specific 5'-Nucleotidase antibody
Anti-Thymidylate 5'-Phosphatase antibody
Anti-Cd Antigen Cd73 antibody
Anti-NT5E antibody
Anti-NT5 antibody
Anti-NTE antibody

Information sourced from Uniprot.org

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