• Western blot analysis of the lysates from K562 cells using ARHGEF5 antibody.
  • Western blot analysis of lysates from RAW264.7 cells, using ARHGEF5 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ARHGEF5 antibody (1280-1330 aa) (STJ94826)

SKU:
STJ94826

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Rho guanine nucleotide exchange factor 5 (1280-1330 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARHGEF5
Gene ID: 7984
Uniprot ID: ARHG5_HUMAN
Immunogen Region: 1280-1330 aa
Specificity: Oncogene TIM Polyclonal Antibody detects endogenous levels of Oncogene TIM protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ARHGEF5 at the amino acid range 1280-1330
Post Translational Modifications Activation of SRC induces tyrosine phosphorylation of ARHGEF5.
Function Guanine nucleotide exchange factor which activates Rho GTPases. Strongly activates RHOA. Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1. Involved in regulation of cell shape and actin cytoskeletal organization. Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia. Required for SRC-induced podosome formation. Involved in positive regulation of immature dendritic cell migration.
Protein Name Rho Guanine Nucleotide Exchange Factor 5
Ephexin-3
Guanine Nucleotide Regulatory Protein Tim
Oncogene Tim
Transforming Immortalized Mammary Oncogene
P60 Tim
Database Links Reactome: R-HSA-193648
Reactome: R-HSA-416482
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013408
Cellular Localisation Cytoplasm
Nucleus
Cell Projection
Podosome
Alternative Antibody Names Anti-Rho Guanine Nucleotide Exchange Factor 5 antibody
Anti-Ephexin-3 antibody
Anti-Guanine Nucleotide Regulatory Protein Tim antibody
Anti-Oncogene Tim antibody
Anti-Transforming Immortalized Mammary Oncogene antibody
Anti-P60 Tim antibody
Anti-ARHGEF5 antibody
Anti-TIM antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance