• Immunohistochemistry analysis of paraffin-embedded human brain, using PARP3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PARP3 antibody (10-59 aa) (STJ94960)

SKU:
STJ94960

Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Protein mono-ADP-ribosyltransferase PARP3 ADP-ribosyltransferase 3 (10-59 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PARP3
Gene ID: 10039
Uniprot ID: PARP3_HUMAN
Immunogen Region: 10-59 aa
Specificity: PARP-3 Polyclonal Antibody detects endogenous levels of PARP-3 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PARP3 at the amino acid range 10-59
Post Translational Modifications Auto-mono-ADP-ribosylated.
Function Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins and plays a key role in the response to DNA damage. Mediates mono-ADP-ribosylation of glutamate, aspartate or lysine residues on target proteins. In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation. Involved in DNA repair by mediating mono-ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism, such as histone H2B, XRCC5 and XRCC6. ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Involved in single-strand break repair by catalyzing mono-ADP-ribosylation of histone H2B on 'Glu-2' (H2BE2ADPr) of nucleosomes containing nicked DNA. Cooperates with the XRCC5-XRCC6 (Ku80-Ku70) heterodimer to limit end-resection thereby promoting accurate NHEJ. Suppresses G-quadruplex (G4) structures in response to DNA damage. Associates with a number of DNA repair factors and is involved in the response to exogenous and endogenous DNA strand breaks. Together with APLF, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ). May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Acts as a negative regulator of immunoglobulin class switch recombination, probably by controlling the level of AICDA /AID on the chromatin. In addition to proteins, also able to ADP-ribosylate DNA: mediates DNA mono-ADP-ribosylation of DNA strand break termini via covalent addition of a single ADP-ribose moiety to a 5'- or 3'-terminal phosphate residues in DNA containing multiple strand breaks.
Protein Name Protein Mono-Adp-Ribosyltransferase Parp3
Adp-Ribosyltransferase Diphtheria Toxin-Like 3
Artd3
Dna Adp-Ribosyltransferase Parp3
Irt1
Nad(+ Adp-Ribosyltransferase 3
Adprt-3
Poly Adp-Ribose Polymerase 3
Parp-3
Hparp-3
PolyAdp-Ribose Synthase 3
Padprt-3
Cellular Localisation Nucleus
Chromosome
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Almost Exclusively Localized In The Nucleus And Appears In Numerous Small Foci And A Small Number Of Larger Foci Whereas A Centrosomal Location Has Not Been Detected
In Response To Dna Damage
Localizes To Sites Of Double-Strand Break
Also Localizes To Single-Strand Breaks
Preferentially Localized To The Daughter Centriole
Alternative Antibody Names Anti-Protein Mono-Adp-Ribosyltransferase Parp3 antibody
Anti-Adp-Ribosyltransferase Diphtheria Toxin-Like 3 antibody
Anti-Artd3 antibody
Anti-Dna Adp-Ribosyltransferase Parp3 antibody
Anti-Irt1 antibody
Anti-Nad(+ Adp-Ribosyltransferase 3 antibody
Anti-Adprt-3 antibody
Anti-Poly Adp-Ribose Polymerase 3 antibody
Anti-Parp-3 antibody
Anti-Hparp-3 antibody
Anti-PolyAdp-Ribose Synthase 3 antibody
Anti-Padprt-3 antibody
Anti-PARP3 antibody
Anti-ADPRT3 antibody
Anti-ADPRTL3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance