• Western blot analysis of extracts of various cell lines, using PEBP1 antibody (STJ24954) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of C6 cells using PEBP1 antibody (STJ24954) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using PEBP1 antibody (STJ24954) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using PEBP1 antibody (STJ24954) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 150ug extracts of MCF-7 cells using 3ug PEBP1 antibody (STJ24954). Western blot was performed from the immunoprecipitate using PEBP1 antibody (STJ24954) at a dilition of 1:500.

Anti-PEBP1 antibody (1-187) (STJ24954)

SKU:
STJ24954

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PEBP1 (1-187) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PEBP1
Gene ID: 5037
Uniprot ID: PEBP1_HUMAN
Immunogen Region: 1-187
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human PEBP1 (NP_002558.1).
Immunogen Sequence: MPVDLSKWSGPLSLQEVDEQ PQHPLHVTYAGAAVDELGKV LTPTQVKNRPTSISWDGLDS GKLYTLVLTDPDAPSRKDPK YREWHHFLVVNMKGNDISSG TVLSDYVGSGPPKGTGLHRY VWLVYEQDRPLKCDEPILSN RSGDHRGKFKVASFRKKYEL RAPVAGTCYQAEWDDYVPKL YEQLSGK
Function Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation. HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.
Protein Name Phosphatidylethanolamine-Binding Protein 1
Pebp-1
Hcnppp
Neuropolypeptide H3
Prostatic-Binding Protein
Raf Kinase Inhibitor Protein
Rkip Cleaved Into - Hippocampal Cholinergic Neurostimulating Peptide
Hcnp
Database Links Reactome: R-HSA-5674135
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Phosphatidylethanolamine-Binding Protein 1 antibody
Anti-Pebp-1 antibody
Anti-Hcnppp antibody
Anti-Neuropolypeptide H3 antibody
Anti-Prostatic-Binding Protein antibody
Anti-Raf Kinase Inhibitor Protein antibody
Anti-Rkip Cleaved Into - Hippocampal Cholinergic Neurostimulating Peptide antibody
Anti-Hcnp antibody
Anti-PEBP1 antibody
Anti-PBP antibody
Anti-PEBP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance