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Anti-Phospho-EGFR (S1071) antibody

[STJ90568] Download PDF Print Data Sheet
Anti-Phospho-EGFR (S1071) antibody

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Product name Anti-Phospho-EGFR (S1071) antibody
Short Description Rabbit Polyclonal to Phospho-EGFR (S1071).
Description EGFR is a protein encoded by the EGFR gene which is approximately 134,3 kDa. EGFR is localised to the cell membrane. and endoplasmic reticulum membrane. It is involved in RET signalling, apoptotic pathways in synovial fibroblasts, glioma and the GPCR pathway. It is a transmembrane glycoprotein that is a member of the protein kinase superfamily. It binds to epidermal growth factor and this induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. EGFR is expressed ubiquitously with isoform 2 also being expressed in ovarian cancers. Mutations in the EGFR gene may result in lung cancer and Cowden syndrome. STJ90568 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous EGFR protein which only binds about S1071 when S1071 is phosphorylated.
Applications ELISA, WB
Dilution range WB 1:500-1:2000
ELISA 1:5000
Specificity Phospho-EGFR (S1071) Polyclonal Antibody detects endogenous levels of EGFR protein only when phosphorylated at S1071.
Protein Name Epidermal growth factor receptor
Proto-oncogene c-ErbB-1
Receptor tyrosine-protein kinase erbB-1
Immunogen Synthesized peptide derived from human EGFR around the phosphorylation site of S1071.
Immunogen Region 1010-1090 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 1956
Gene Symbol EGFR
Molecular Weight 150-160 kDa
Database Links HGNC:3236
OMIM:131550
Alternative Names EGFR antibody
ERBB antibody
ERBB1 antibody
HER1 antibody
Epidermal growth factor receptor antibody
Proto-oncogene c-ErbB-1 antibody
Receptor tyrosine-protein kinase erbB-1 antibody
Phospho-EGFR (S1071) antibody
Function Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Plays a role in enhancing learning and memory performance (By similarity). ; Isoform 2 may act as an antagonist of EGF action.; (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.
Post-translational Modifications Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2. ; Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126 (By similarity). ; Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
Cellular Localization Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).; Isoform 2: Secreted.
Tissue Specificity Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.

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