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Anti-Phospho-ERK 1/2-Thr202 antibody (169-218 aa) (STJ90260)

Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of KB cells using Phospho-ERK 1/2 (T202) Polyclonal Antibody diluted at 1：1000
Immunofluorescence analysis of mouse-liver tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-colon-cancer tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of mouse-spleen tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of mouse-spleen tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of various cells using Phospho-ERK 1/2 (T202) Polyclonal Antibody diluted at 1：1000
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of lysates from NIH/3T3 cells treated with IFN 2500U/ml 30', using p44/42 MAP Kinase (Phospho-Thr202) Antibody. The lane on the right is blocked with the phospho peptide.
Immunofluorescence analysis of mouse-liver tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-ERK 1/2-Thr202 antibody (169-218 aa) (STJ90260)

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STJ90260

£46.00 - £226.00

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General Information
Product Properties
Target Information
Additional Info
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Host:	Rabbit
Applications:	IF/WB/IHC/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Phospho-Mitogen-activated protein kinase 3 and Mitogen-activated protein kinase 1-Thr202 (169-218 aa) is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	IF 1:50-200 WB 1:500-2000 IHC 1:50-300 IHC 1:50-300
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	MAPK1 MAPK3
Gene ID:	5594 5595
Uniprot ID:	MK01_HUMAN MK03_HUMAN
Immunogen Region:	169-218 aa
Specificity:	Phospho-ERK 1/2 (T202) Polyclonal Antibody detects endogenous levels of ERK 1/2 protein only when phosphorylated at T202.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human p44/42 MAP Kinase around the phosphorylation site of Thr202 at the amino acid range 169-218

Function	Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC).as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Protein Name	Mitogen-Activated Protein Kinase 3 Map Kinase 3 Mapk 3 Ert2 Extracellular Signal-Regulated Kinase 1 Erk-1 Insulin-Stimulated Map2 Kinase Map Kinase Isoform P44 P44-Mapk Microtubule-Associated Protein 2 Kinase P44-Erk1
Database Links	Reactome: R-HSA-110056 Reactome: R-HSA-112409 Reactome: R-HSA-1169408 Reactome: R-HSA-1295596 Reactome: R-HSA-162658 P27361-3 Reactome: R-HSA-170968 Reactome: R-HSA-198753 Reactome: R-HSA-202670 Reactome: R-HSA-2029482 Reactome: R-HSA-2559580 Reactome: R-HSA-2559582 Reactome: R-HSA-2559585 Reactome: R-HSA-2871796 Reactome: R-HSA-3371453 Reactome: R-HSA-375165 Reactome: R-HSA-444257 Reactome: R-HSA-445144 Reactome: R-HSA-450341 Reactome: R-HSA-456926 Reactome: R-HSA-5654726 Reactome: R-HSA-5654727 Reactome: R-HSA-5654732 Reactome: R-HSA-5654733 Reactome: R-HSA-5663213 Reactome: R-HSA-5668599 Reactome: R-HSA-5673001 Reactome: R-HSA-5674135 Reactome: R-HSA-5674499 Reactome: R-HSA-5675221 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-6811558 Reactome: R-HSA-73728 Reactome: R-HSA-74749 Reactome: R-HSA-879415 Reactome: R-HSA-881907 Reactome: R-HSA-8939211 Reactome: R-HSA-8940973 Reactome: R-HSA-8943724 Reactome: R-HSA-9627069 Reactome: R-HSA-9634638 Reactome: R-HSA-9635465 Reactome: R-HSA-9649948 Reactome: R-HSA-9652169 Reactome: R-HSA-9656223 Reactome: R-HSA-9664422 Reactome: R-HSA-982772
Cellular Localisation	Cytoplasm Nucleus Membrane Caveola Autophosphorylation At Thr-207 Promotes Nuclear Localization
Alternative Antibody Names	Anti-Mitogen-Activated Protein Kinase 3 antibody Anti-Map Kinase 3 antibody Anti-Mapk 3 antibody Anti-Ert2 antibody Anti-Extracellular Signal-Regulated Kinase 1 antibody Anti-Erk-1 antibody Anti-Insulin-Stimulated Map2 Kinase antibody Anti-Map Kinase Isoform P44 antibody Anti-P44-Mapk antibody Anti-Microtubule-Associated Protein 2 Kinase antibody Anti-P44-Erk1 antibody Anti-MAPK3 antibody Anti-ERK1 antibody Anti-PRKM3 antibody

Information sourced from Uniprot.org

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