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Anti-Phospho-ERK 1/2 (Y222/205) antibody

[STJ90657] Download PDF Print Data Sheet
5-7 days

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Product name Anti-Phospho-ERK 1/2 (Y222/205) antibody
Short Description Rabbit polyclonal to Phospho-ERK 1/2 (Y222/205).
Description ERK 1/2 is a protein encoded by the MAPK1 gene which is approximately 41,4 kDa. ERK 1/2 is localised to the cytoplasm and nucleus. It is involved in RET signalling, activated TLR4 signalling, IL-2 pathway, regulation of lipid metabolism and insulin signalling-generic cascades. This protein falls under the MAP kinase family. It is a serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. It also acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. ERK 1/2 is expressed in the nervous system, blood, lung, liver and skin. Mutations in the MAPK1 gene may result in small intestine neuroendocrine neoplasm and pertussis. STJ90657 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous ERK 1/2 protein which only binds about Y222/205 when Y222/205 is phosphorylated.
Applications ELISA, WB
Dilution range WB 1:500-1:2000
ELISA 1:10000
Specificity Phospho-ERK 1/2 (Y222/205) Polyclonal Antibody detects endogenous levels of ERK 1/2 protein only when phosphorylated at Y222/205.
Protein Name Mitogen-activated protein kinase 1
MAP kinase 1
MAPK 1
ERT1
Extracellular signal-regulated kinase 2
ERK-2
MAP kinase isoform p42
p42-MAPK
Mitogen-activated protein kinase 2
MAP kinase 2
MAPK 2
Immunogen Synthesized peptide derived from human ERK 1/2 around the phosphorylation site of Y222/205.
Immunogen Region 140-220 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Mouse, Rat, Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 5594
Gene Symbol MAPK1
Molecular Weight 44/42 kDa
Database Links HGNC:6871
OMIM:176948
Alternative Names Phospho-ERK 1/2 (Y222/205)
Mitogen-activated protein kinase 1 antibody
MAP kinase 1 antibody
MAPK 1 antibody
ERT1 antibody
Extracellular signal-regulated kinase 2 antibody
ERK-2 antibody
MAP kinase isoform p42 antibody
p42-MAPK antibody
Mitogen-activated protein kinase 2 antibody
MAP kinase 2 antibody
MAPK 2 antibody
ERK2 antibody
PRKM1 antibody
PRKM2 antibody
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Phosphorylates CDK2AP2 . Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
Post-translational Modifications Phosphorylated upon KIT and FLT3 signaling . Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Undergoes regulatory phosphorylation on additional residues such as Ser-246 and Ser-248 in the kinase insert domain (KID) These phosphorylations, which are probably mediated by more than one kinase, are important for binding of MAPK1/ERK2 to importin-7 (IPO7) and its nuclear translocation. In addition, autophosphorylation of Thr-190 was shown to affect the subcellular localization of MAPK1/ERK2 as well. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-187. Phosphorylation on Ser-29 by SGK1 results in its activation by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. DUSP3 and DUSP6 dephosphorylate specifically MAPK1/ERK2 and MAPK3/ERK1 whereas DUSP9 dephosphorylates a broader range of MAPKs. ISGylated.
Cellular Localization Cytoplasm, cytoskeleton, spindle Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Membrane, caveola. Associated with the spindle during prometaphase and metaphase . PEA15-binding and phosphorylated DAPK1 promote its cytoplasmic retention. Phosphorylation at Ser- 246 and Ser-248 as well as autophosphorylation at Thr-190 promote nuclear localization.
Sequence and Domain Family The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Swiss-Prot Key P28482_HUMAN
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