• Western blot analysis of extracts of various cell lines, using POMC antibody (STJ25054) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-POMC antibody (1-100) (STJ25054)

SKU:
STJ25054

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POMC (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POMC
Gene ID: 5443
Uniprot ID: COLI_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human POMC (NP_000930.1).
Immunogen Sequence: MPRSCCSRSGALLLALLLQA SMEVRGWCLESSQCQDLTTE SNLLECIRACKPDLSAETPM FPGNGDEQPLTENPRKYVMG HFRWDRFGRRNSSSSGSSGA
Tissue Specificity ACTH and MSH are produced by the pituitary gland.
Post Translational Modifications Specific enzymatic cleavages at paired basic residues yield the different active peptides. O-glycosylated.reducing sugar is probably N-acetylgalactosamine.
Function Corticotropin: Stimulates the adrenal glands to release cortisol. Melanocyte-stimulating hormone alpha: Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes. Melanocyte-stimulating hormone beta: Increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin: Endogenous orexigenic opiate. Met-enkephalin: Endogenous opiate.
Protein Name Pro-Opiomelanocortin
Pomc
Corticotropin-Lipotropin Cleaved Into - Npp - Melanotropin Gamma
Gamma-Msh - Potential Peptide - Corticotropin
Adrenocorticotropic Hormone
Acth - Melanocyte-Stimulating Hormone Alpha
Alpha-Msh
Melanotropin Alpha - Corticotropin-Like Intermediary Peptide
Clip - Lipotropin Beta
Beta-Lph - Lipotropin Gamma
Gamma-Lph - Melanocyte-Stimulating Hormone Beta
Beta-Msh
Melanotropin Beta - Beta-Endorphin - Met-Enkephalin
Database Links Reactome: R-HSA-111885
Reactome: R-HSA-193048
Reactome: R-HSA-194002
Reactome: R-HSA-202040
Reactome: R-HSA-209952
Reactome: R-HSA-211976
Reactome: R-HSA-375276
Reactome: R-HSA-418555
Reactome: R-HSA-418594
Reactome: R-HSA-5579031
Reactome: R-HSA-6785807
Reactome: R-HSA-9615017
Cellular Localisation Secreted
Melanocyte-Stimulating Hormone Alpha And Beta-Endorphin Are Stored In Separate Granules In Hypothalamic Pomc Neurons
Suggesting That Secretion May Be Under The Control Of Different Regulatory Mechanisms
Alternative Antibody Names Anti-Pro-Opiomelanocortin antibody
Anti-Pomc antibody
Anti-Corticotropin-Lipotropin Cleaved Into - Npp - Melanotropin Gamma antibody
Anti-Gamma-Msh - Potential Peptide - Corticotropin antibody
Anti-Adrenocorticotropic Hormone antibody
Anti-Acth - Melanocyte-Stimulating Hormone Alpha antibody
Anti-Alpha-Msh antibody
Anti-Melanotropin Alpha - Corticotropin-Like Intermediary Peptide antibody
Anti-Clip - Lipotropin Beta antibody
Anti-Beta-Lph - Lipotropin Gamma antibody
Anti-Gamma-Lph - Melanocyte-Stimulating Hormone Beta antibody
Anti-Beta-Msh antibody
Anti-Melanotropin Beta - Beta-Endorphin - Met-Enkephalin antibody
Anti-POMC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance