• Western blot analysis of extracts of various cell lines, using RAB27B antibody (STJ112425) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RAB27B antibody (1-218) (STJ112425)

SKU:
STJ112425

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAB27B (1-218) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB27B
Gene ID: 5874
Uniprot ID: RB27B_HUMAN
Immunogen Region: 1-218
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human RAB27B (NP_004154.2).
Immunogen Sequence: MTDGDYDYLIKLLALGDSGV GKTTFLYRYTDNKFNPKFIT TVGIDFREKRVVYNAQGPNG SSGKAFKVHLQLWDTAGQER FRSLTTAFFRDAMGFLLMFD LTSQQSFLNVRNWMSQLQAN AYCENPDIVLIGNKADLPDQ REVNERQARELADKYGIPYF ETSAATGQNVEKAVETLLDL IMKRMEQCVEKTQIPDTVNG GNSGNLDGEKPPEKKCIC
Tissue Specificity Expressed primarily in testis.
Function Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in NTRK2/TRKB axonal anterograde transport by facilitating the association of NTRK2/TRKB with KLC1. May be involved in targeting uroplakins to urothelial apical membranes.
Protein Name Ras-Related Protein Rab-27b
C25kg
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Membrane
Lipid-Anchor
Late Endosome
Alternative Antibody Names Anti-Ras-Related Protein Rab-27b antibody
Anti-C25kg antibody
Anti-RAB27B antibody

Information sourced from Uniprot.org

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