• Western blot analysis of the lysates from HepG2 cells using SMO antibody.
  • Western blot analysis of lysates from HUVEC cells, using SMO Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-SMO antibody (68-117 aa) (STJ95710)

SKU:
STJ95710

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Protein smoothened (68-117 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMO
Gene ID: 6608
Uniprot ID: SMO_HUMAN
Immunogen Region: 68-117 aa
Specificity: Smo Polyclonal Antibody detects endogenous levels of Smo protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human SMO at the amino acid range 68-117
Function G protein-coupled receptor which associates with the patched protein (PTCH) to transduce hedgehog protein signaling. Binding of sonic hedgehog (SHH) to its receptor patched prevents inhibition of smoothened (SMO) by patched. When active, SMO binds to and sequesters protein kinase A catalytic subunit PRKACA at the cell membrane, preventing PRKACA-mediated phosphorylation of GLI transcription factors which releases the GLI proteins from PRKACA-mediated inhibition and allows for transcriptional activation of hedgehog pathway target genes. Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation.
Protein Name Protein Smoothened
Protein Gx
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-5610787
Reactome: R-HSA-5620922
Reactome: R-HSA-5632684
Reactome: R-HSA-5635838
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Projection
Cilium
Cilium Localization Is Promoted By Shh And Is Required For Activity
Alternative Antibody Names Anti-Protein Smoothened antibody
Anti-Protein Gx antibody
Anti-SMO antibody
Anti-SMOH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance