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Anti-Active Caspase-3 antibody

[STJ97448] Download PDF Print Data Sheet

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Product name Anti-Active Caspase-3 antibody
Short Description Mouse Monoclonal to Active Caspase-3.
Description Active caspase-3 is a protein encoded by the CASP3 gene which is approximately 31,6 kDa. Active caspase-3 is localised to the cytoplasm. It is involved in the TNFR1 pathway, apoptosis modulation and signalling and respiratory electron transport. This protein falls under the cysteine-aspartic acid protease family. It is a sequential activator of caspases and plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Active caspase-3 is highly expressed in lung, spleen, heart, liver and kidney. Mutations in the CASP3 gene may result in bladder urothelial carcinoma and primary effusion lymphoma. STJ97448 was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. This antibody detects endogenous active caspase-3 protein.
Applications IHC-p, IF, WB
Dilution range WB 1:500-1000
IHC 1:100-200
Specificity The antibody detects endogenous Active Caspase-3 protein.
Protein Name Caspase-3
Cysteine protease CPP32
Protein Yama
SREBP cleavage activity 1
Caspase-3 subunit p17
Caspase-3 subunit p12
Immunogen Recombinant Protein
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Mouse
Clonality Monoclonal
Clone ID 5E1
Reactivity Chicken, Human, Mouse, Rat
Conjugation Unconjugated
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Isotype IgG1
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 836
Gene Symbol CASP3
Database Links HGNC:1504
Alternative Names CASP3 antibody
CPP32 antibody
Caspase-3 antibody
CASP-3 antibody
Apopain antibody
Cysteine protease CPP32 antibody
CPP-32 antibody
Protein Yama antibody
SREBP cleavage activity 1 antibody
SCA-1 antibody
Caspase-3 subunit p17 antibody
Caspase-3 subunit p12 antibody
Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Post-translational Modifications Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.; S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
Cellular Localization Cytoplasm.
Tissue Specificity Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Swiss-Prot Key P42574_HUMAN


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