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Anti-Active Caspase-3 antibody

[STJ97448] Download PDF Print Data Sheet

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Product name Anti-Active Caspase-3 antibody
Short Description Mouse Monoclonal to Active Caspase-3.
Description Active caspase-3 is a protein encoded by the CASP3 gene which is approximately 31,6 kDa. Active caspase-3 is localised to the cytoplasm. It is involved in the TNFR1 pathway, apoptosis modulation and signalling and respiratory electron transport. This protein falls under the cysteine-aspartic acid protease family. It is a sequential activator of caspases and plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Active caspase-3 is highly expressed in lung, spleen, heart, liver and kidney. Mutations in the CASP3 gene may result in bladder urothelial carcinoma and primary effusion lymphoma. STJ97448 was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. This antibody detects endogenous active caspase-3 protein.
Applications IHC-p, IF, WB
Dilution range WB 1:500-1000 / IHC 1:100-200
Specificity The antibody detects endogenous Active Caspase-3 protein.
Protein Name Caspase-3 - CASP3 - CPP32 - CPP32B - Yama - apopain - caspase 3
Immunogen Recombinant Protein
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Mouse
Clonality Monoclonal
Clone ID 5E1
Reactivity Chicken, Human, Mouse, Rat
Conjugation Unconjugated
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Isotype IgG1
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 836 (Human) /
Gene Symbol CASP3
Database Links Human UniProt/Swiss-Prot:P42574 Mouse UniPort/Swiss-Prot: P70677 Rat UniProt/Swiss-Port: P55213 Human Entrez Gene: 836 Mouse Entrez Gene: 12367 Rat Entrez Gene: Rn.10562
Alternative Names CASP3 / caspase 3 / AC-3 antibody / Apopain antibody / CASP-3 antibody / casp3 protein antibody / Caspase-3 antibody / caspase 3A antibody / caspase 3 / apoptosis-related cysteine peptidase antibody / caspase 3 / apoptosis related cysteine protease antibody / caspase 3 / apoptosis-related cysteine protease antibody / Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease) antibody / Caspase 3 / apoptosis related cysteine protease (ICE-like cysteine protease) antibody / caspase 3-like antibody / Caspase-3-like protein antibody / caspase-3-like protein 1 antibody / caspase-3 preproprotein antibody / CB1_000998003 antibody / CC3 antibody / cpp32 antibody / CPP-32 antibody / CPP32B antibody / CPP32beta antibody / Cysteine protease CPP32 antibody / GW7_02720 antibody / H920_14272 antibody / hypothetical protein antibody / IRP antibody / Lice antibody / MDA_GLEAN10021003 antibody / mldy antibody / PAL_GLEAN10021656 antibody / PANDA_001909 antibody / PARP cleavage protease antibody / procaspase3 antibody / Protein Yama antibody / SCA-1 antibody / SREBP cleavage activity 1 antibody / UY3_01982 antibody / xCPP32 antibody / Y1Q_030565 antibody / Yama antibody / yama protein antibody
Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Post-translational Modifications Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.; S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
Cellular Localization Cytoplasm.
Tissue Specificity Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

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