SHOPPING BASKET: 0 item(s) - £0.00  

You have no items in your shopping cart.

Anti-Akt1 antibody

[STJ91542] Download PDF Print Data Sheet

* Required Fields

£0.00
Product name Anti-Akt1 antibody
Description Rabbit Polyclonal to Akt1.
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC, WB
Immunogen Synthesized peptide derived from human Akt1 around the non-phosphorylation site of S129.
Gene ID 207 (Human) / 11651 (Mouse) / 24185 (Rat)
Dilution range WB 1:500-1:2000 / IHC 1:100-1:300 / IF 1:200-1:1000 / ELISA 1:40000 /
Specificity Akt1 Polyclonal Antibody detects endogenous levels of Akt1 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Note For Research Use Only (RUO).
Protein Name RAC-alpha serine/threonine-protein kinase - AKT1 - RAC - PKB - PRKBA - AKT - AKT serine/threonine kinase 1
Clonality Polyclonal
Conjugation Unconjugated
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration 1 mg/ml
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Database Links Human UniProt/Swiss-Prot:P31749 Mouse UniPort/Swiss-Prot: P31750 Rat UniProt/Swiss-Port: P47196 Human Entrez Gene: 207 Mouse Entrez Gene: 11651 Rat Entrez Gene: Rn.11422
Alternative Names AKT1 / v-akt murine thymoma viral oncogene homolog 1 / AKT antibody / akt-1 antibody / AKT1 kinase antibody / AKT1m antibody / Akt1m protein antibody / Akt1-PA antibody / Akt1-PB antibody / Akt1-PC antibody / Akt1-PD antibody / Akt1-PE antibody / Akt kinase antibody / AKT kinase-transforming protein antibody / AKT kinase-transforming protein-like protein antibody / AKT /PKB antibody / Anapl_13822 antibody / AS27_04659 antibody / AS28_02100 antibody / CG4006 gene product from transcript CG4006-RE antibody / CG4006-PA antibody / CG4006-PB antibody / CG4006-PC antibody / CG4006-PD antibody / CG4006-PE antibody / CWS6 antibody / Dakt antibody / D-Akt antibody / DAKT1 antibody / DAKT1 /PKB antibody / dAKT /dPKB antibody / dAkt kinase antibody / dAkt /PKB antibody / Dmel_CG4006 antibody / DPKB antibody / DRAC-PK antibody / DRAC-PK66 antibody / DRAC-PK85 antibody / EGK_18599 antibody / GB15685 antibody / H920_16329 antibody / M959_12657 antibody / murine thymoma viral (v-akt) oncogene homolog 1 antibody / N300_11597 antibody / N301_15140 antibody / N302_00176 antibody / N303_00746 antibody / N306_05594 antibody / N307_03832 antibody / N308_01640 antibody / N309_08822 antibody / N320_00776 antibody / N321_12980 antibody / N322_01881 antibody / N324_06306 antibody / N327_12383 antibody / N328_12348 antibody / N330_13811 antibody / N334_00883 antibody / N335_11264 antibody / N336_09837 antibody / N339_03367 antibody / N340_12456 antibody / pAkt antibody / p-Akt antibody / PAL_GLEAN10020954 antibody / PKB antibody / PKB /Akt antibody / PKBalpha antibody / PKB alpha antibody / PKB-ALPHA antibody / PKB /dAKT antibody / PRKBA antibody / protein kinase Akt-1 antibody / protein kinase B antibody / protein kinase B alpha antibody / protein kinase B-alpha antibody / protein kinase B / alpha antibody / proto-oncogene c-Akt antibody / RAC antibody / RAC-ALPHA antibody / RAC-alpha serine /threonine-protein kinase antibody / RacPK antibody / RAC-PK-alpha antibody / rac protein kinase alpha antibody / RAC protein kinase alpha RAC-PK alpha antibody / RAC serine /threonine-protein kinase antibody / related to A and C kinases antibody / related to PKA to PKC protein kinases antibody / related to the A and C kinases antibody / serine-threonine protein kinase antibody / serine /threonine protein kinase antibody / thymoma viral proto-oncogene 1 antibody / UY3_14824 antibody / v-akt antibody / v-akt1 antibody / v-akt murine thymoma viral oncogene-like 1 antibody / v-akt murine thymoma viral oncogene-like protein 1 antibody / v-akt murine thymoma viral oncoprotein-like protein 1 antibody / xAct antibody / xAkt antibody / Y1Q_005191 antibody / Y956_11654 antibody / Z169_03636 antibody
Function AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI(3)P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development. Phosphorylates STK4/MST1 at 'Thr-120' and 'Thr-387' leading to inhibition of its: kinase activity, nuclear translocation, autophosphorylation and ability to phosphorylate FOXO3. Phosphorylates STK3/MST2 at 'Thr-117' and 'Thr-384' leading to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation. Phosphorylates RAF1 at 'Ser-259' and negatively regulates its activity. Phosphorylation of BAD stimulates its pro-apoptotic activity. Phosphorylates KAT6A at 'Thr-369' and this phosphorylation inhibits the interaction of KAT6A with PML and negatively regulates its acetylation activity towards p53/TP53.; AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.
Sequence and Domain Family Binding of the PH domain to phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P3) following phosphatidylinositol 3-kinase alpha (PIK3CA) activity results in its targeting to the plasma membrane. The PH domain mediates interaction with TNK2 and Tyr-176 is also essential for this interaction.; The AGC-kinase C-terminal mediates interaction with THEM4.
Post-translational Modifications O-GlcNAcylation at Thr-305 and Thr-312 inhibits activating phosphorylation at Thr-308 via disrupting the interaction between AKT1 and PDPK1. O-GlcNAcylation at Ser-473 also probably interferes with phosphorylation at this site. ; Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Phosphorylated at Thr-308 and Ser-473 by IKBKE and TBK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells. Ser-473 phosphorylation is enhanced by signaling through activated FLT3. Dephosphorylated at Thr-308 and Ser-473 by PP2A phosphatase. The phosphorylated form of PPP2R5B is required for bridging AKT1 with PP2A phosphatase. Ser-473 is dephosphorylated by CPPED1, leading to termination of signaling. ; Ubiquitinated via 'Lys-48'-linked polyubiquitination by ZNRF1, leading to its degradation by the proteasome (By similarity). Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. Also ubiquitinated by TRIM13 leading to its proteasomal degradation. Phosphorylated, undergoes 'Lys-48'-linked polyubiquitination preferentially at Lys-284 catalyzed by MUL1, leading to its proteasomal degradation. ; Acetylated on Lys-14 and Lys-20 by the histone acetyltransferases EP300 and KAT2B. Acetylation results in reduced phosphorylation and inhibition of activity. Deacetylated at Lys-14 and Lys-20 by SIRT1. SIRT1-mediated deacetylation relieves the inhibition.
Cellular Localization Cytoplasm. Nucleus. Cell membrane. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles .

Tags:

Use spaces to separate tags. Use single quotes (') for phrases.

Bad Good
Enter the code in the box below: