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Anti-β-actin antibody

[STJ96930] Download PDF Print Data Sheet

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Product name Anti-β-actin antibody
Description Mouse Monoclonal to β-actin.
Host Mouse
Reactivity Chicken, Dog, Hamster, Human, Insect, Monkey, Mouse, Rabbit, Rat
Applications ICC, IHC, WB
Immunogen Synthetic Peptide
Gene ID 60 (Human) / 11461 (Mouse) / 81822 (Rat)
Dilution range WB 1:5000 / IHC 1:200
Specificity The antibody detects endogenous β-actin protein.
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Clone ID 5B7
Note For Research Use Only (RUO).
Protein Name Actin cytoplasmic 1 - ACTB - actin beta
Clonality Monoclonal
Conjugation Unconjugated
Isotype IgG1
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration 1 mg/ml
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Database Links Human UniProt/Swiss-Prot:P60709 Mouse UniPort/Swiss-Prot: P60710 Rat UniProt/Swiss-Port: P60711 Human Entrez Gene: 60 Mouse Entrez Gene: 11461 Rat Entrez Gene: Rn.94978
Alternative Names ACTB / actin / beta / act-2 antibody / ACT-5 antibody / ACTA1 antibody / actba antibody / actin antibody / actin / alpha 1 / skeletal muscle antibody / actin beta antibody / actin / beta / cytoplasmic antibody / Actin / cytoplasmic 1 antibody / Actin / cytoplasmic type 5 antibody / Actx antibody / Anapl_00512 antibody / AS27_12274 antibody / AS28_05902 antibody / A-X actin-like protein antibody / Bact antibody / Bact / actin antibody / B-actin antibody / beta actin antibody / Beta-actin antibody / beta actin1 antibody / Beta-actin A antibody / beta cytoskeletal actin antibody / BRWS1 antibody / cytoplasmic 1 antibody / cytoplasmic actin OlCA1 antibody / cytoplasmic beta actin antibody / cytoplasmic beta-actin antibody / cytoskeletal beta actin antibody / D623_10011024 antibody / gamma non-muscle actin antibody / GW7_16883 antibody / H920_13304 antibody / hypothetical protein antibody / M91_16383 antibody / M959_04960 antibody / MDA_GLEAN10021467 antibody / melanoma X-actin antibody / N300_09809 antibody / N301_15340 antibody / N302_11832 antibody / N303_00828 antibody / N305_14864 antibody / N306_14056 antibody / N307_07714 antibody / N308_12235 antibody / N309_01588 antibody / N312_00100 antibody / olCA1 antibody / PAL_GLEAN10018968 antibody / PANDA_004593 antibody / PS1TP5-binding protein 1 antibody / ps1tp5bp1 antibody / putative beta-actin antibody / TREES_T100021367 antibody / UY3_09776 antibody / Y1Q_025431 antibody / Y956_00599 antibody / Z169_07136 antibody
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Post-translational Modifications ISGylated. ; Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). ; Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. ; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding . The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners .
Cellular Localization Cytoplasm, cytoskeleton Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.


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