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Anti-β-Tubulin antibody

[STJ96932] Download PDF Print Data Sheet
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Product name Anti-β-Tubulin antibody
Short Description Mouse Monoclonal to β-Tubulin.
Description Beta tubulin is a protein encoded by the tubb gene which is approximately 49,7 kDa. Beta tubulin is localised to the cytoskeleton and cytoplasm. It is involved in the regulation of PLK1 activity at G2/M transition, development of Slit-Robo signalling, the innate immune system, cell cycle and organelle biogenesis and maintenance. Beta tubulin contains a highly acidic C-terminal region which can bind cations such as calcium. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain and forms part of the cytoskeleton. Beta tubulin is ubiquitously expressed in the spleen, thymus and immature brain. Mutations in the tubb gene result in complex cortical dysplasia with other brain malformations. Mutations can also cause congenital symmetric circumferential, an autosomal dominant disease which results in multiple rings of folded skin mostly affecting the limbs. STJ96932 was developed from clone 1E1-E8-H4 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous beta tubulin.
Applications IHC-p, WB
Dilution range WB 1:5000
IHC 1:200
Specificity The antibody detects endogenous β-tubulin protein.
Protein Name Tubulin beta-3 chain
Tubulin beta-4 chain
Tubulin beta-III
Immunogen Synthetic Peptide
Immunogen Region C-term
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Mouse
Clonality Monoclonal
Clone ID 5G3
Reactivity Chicken, Dog, Hamster, Human, Insect, Monkey, Mouse, Rabbit, Rat, Sheep, Yeast
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Isotype IgG1
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 10381
Gene Symbol TUBB3
Database Links HGNC:20772
OMIM:600638
Alternative Names TUBB3 antibody
TUBB4 antibody
Tubulin beta-3 chain antibody
Tubulin beta-4 chain antibody
Tubulin beta-III antibody
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Post-translational Modifications Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group . Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold . ; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). ; Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
Cellular Localization Cytoplasm, cytoskeleton.
Tissue Specificity Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.
Sequence and Domain Family The highly acidic C-terminal region may bind cations such as calcium.
Swiss-Prot Key Q13509_HUMAN
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1
I am interested in this antibody in 0.3mg size, 1mg/mL. Would it be possible to change the storage buffer to PBS and not include sodium azide?
Question by: Guest on 11 Apr 2018 13:40:00
-3
Hello, thank you for your query. If you would like to place a custom order, the product should be at least 1 mg. There will be no additional cost for changing the storage buffer. Please contact us at info@stjohnslabs.com for further information.
Answer by: Customer Support on 11 Apr 2018 13:43:00

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