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Anti-β-tubulin antibody

[STJ97037] Download PDF Print Data Sheet

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Product name Anti-β-tubulin antibody
Short Description Mouse Monoclonal to β-tubulin.
Description Beta tubulin is a protein encoded by the tubb gene which is approximately 49,7 kDa. Beta tubulin is localised to the cytoskeleton and cytoplasm. It is involved in the regulation of PLK1 activity at G2/M transition, development of Slit-Robo signalling, the innate immune system, cell cycle and organelle biogenesis and maintenance. Beta tubulin contains a highly acidic C-terminal region which can bind cations such as calcium. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain and forms part of the cytoskeleton. Beta tubulin is ubiquitously expressed in the spleen, thymus and immature brain. Mutations in the tubb gene result in complex cortical dysplasia with other brain malformations. Mutations can also cause congenital symmetric circumferential, an autosomal dominant disease which results in multiple rings of folded skin mostly affecting the limbs. STJ97037 was developed from clone M7. The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous beta tubulin.
Applications IF, WB
Dilution range WB 1:5000 -10000 / IF 1:100-200
Specificity The antibody detects endogenousβ-tubulin protein.
Protein Name Tubulin beta-3 chain - TUBB3 - beta-4 - CFEOM3 - CFEOM3A - FEOM3 - tubulin beta 3 class III
Immunogen Synthetic Peptide
Immunogen Region C-term
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Mouse
Clonality Monoclonal
Clone ID M7
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Isotype IgG1
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 10381 (Human) / 22152 (Mouse) / 246118 (Rat)
Gene Symbol TUBB3
Database Links Human UniProt/Swiss-Prot:Q13509 Mouse UniPort/Swiss-Prot: Q9ERD7 Rat UniProt/Swiss-Port: Q4QRB4 Human Entrez Gene: 10381 Mouse Entrez Gene: 22152 Rat Entrez Gene: Rn.43958
Alternative Names TUBB3 / tubulin beta 3 class III / A306_01682 antibody / AS27_07488 antibody / beta-4 antibody / beta-4-tubulin antibody / betaIII-tubulin antibody / beta-tubulin class-III antibody / CDCBM antibody / CDCBM1 antibody / CFEOM3 antibody / CFEOM3A antibody / class III beta-tubulin antibody / D623_10003790 antibody / FEOM3 antibody / GW7_12416 antibody / H920_06773 antibody / hypothetical protein antibody / I79_000194 antibody / M(beta)3 antibody / M(beta)6 antibody / MC1R antibody / melanocortin 1 receptor antibody / melanocortin-1 receptor antibody / Melanocyte-stimulating hormone receptor antibody / N300_09138 antibody / N301_08954 antibody / N302_09664 antibody / N303_10590 antibody / N305_04768 antibody / N308_02301 antibody / neuron-specific class III beta-tubulin antibody / PANDA_016558 antibody / QccE-15186 antibody / TREES_T100001682 antibody / TUBB4 antibody / tubulin beta3 antibody / tubulin / beta / 3 antibody / Tubulin beta-3 chain antibody / tubulin / beta 3 class III antibody / tubulin / beta / 4 antibody / Tubulin beta-4 chain antibody / tubulin beta-III antibody / UY3_00776 antibody / Y956_10968 antibody
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Post-translational Modifications Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group . Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold . ; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). ; Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
Cellular Localization Cytoplasm, cytoskeleton.
Tissue Specificity Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.
Sequence and Domain Family The highly acidic C-terminal region may bind cations such as calcium.

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