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Anti-BrdU antibody

[STJ22838] Download PDF Print Data Sheet
Anti-BrdU antibody

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Product name Anti-BrdU antibody
Short Description Mouse Monoclonal against BrdU protein
Description The halogenated pyrimidine thymidine analog bromodeoxyuridine (BrdU) is incorporated into newly synthesized DNA strands of S phase cells and is useful for estimating the fraction of cells in S phase. Additionally, the analysis of the uptake of BrdU is a reliable method to quantitate the degree of DNA synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. Anti-BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with cross-linking agents such as formalin but may decrease the specificity of BrdU immunodetection. The monoclonal antibody Bu20a against BrdU stains BrdU incorporated into the nulcei of a wide range of proliferating cell types including human tumors growing in nude mice and tonsil lymphoid.
Applications ICC, IF, IHC
Dilution range IHC 1:50-1:500
IF 1:50-1:500
FC 1:50-1:500
Protein Name BrdU
Immunogen Bromodeoxyuridine.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Mouse
Clonality Monoclonal
Reactivity ALL
Conjugation Unconjugated
Concentration 1 mg/ml
Purification Anti-BrdU antibody was affinity purified using immunizing peptide
Isotype IgG1
Formulation PBS with 0.02% sodium azide, 50% glycerol, pH7.4
Alternative Names BrdU antibody

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