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Anti-Caspase 9 antibody

[STJ96979] Download PDF Print Data Sheet
5-7 days

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Product name Anti-Caspase 9 antibody
Short Description Mouse Monoclonal to Caspase 9.
Description Caspase-9 is a protein encoded by the CASP9 gene which is approximately 46,2 kDa. Caspase-9 is localised to the cytosol, mitochondrion and nucleus. It is involved in the TNFR1 pathway, RET signalling and apoptosis modulation and signalling. Caspase-9 exist as an inactive proenzyme which undergoes proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. It is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase-9 is ubiquitously expressed with highest expression in the heart. Mutations in the CASP9 gene may result in cerebral hypoxia and cervical cancer. STJ96979 was affinity purified. This monoclonal antibody binds endogenous caspase-9.
Applications IP, WB
Dilution range WB 1:1000-5000
IP 1:200
Specificity The antibody detects endogenous Caspase 9 protein.
Protein Name Caspase-9
CASP-9
Apoptotic protease Mch-6
Apoptotic protease-activating factor 3
APAF-3
ICE-like apoptotic protease 6
ICE-LAP6
Caspase-9 subunit p35
Caspase-9 subunit p10
Immunogen Synthetic Peptide
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Mouse
Clonality Monoclonal
Clone ID 3-20
Reactivity Chicken, Human, Mouse, Rat
Conjugation Unconjugated
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Isotype IgG1
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 842
Gene Symbol CASP9
Database Links HGNC:1511
OMIM:602234
Alternative Names CASP9 antibody
MCH6 antibody
Caspase-9 antibody
CASP-9 antibody
Apoptotic protease Mch-6 antibody
Apoptotic protease-activating factor 3 antibody
APAF-3 antibody
ICE-like apoptotic protease 6 antibody
ICE-LAP6 antibody
Caspase-9 subunit p35 antibody
Caspase-9 subunit p10 antibody
Function Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).; Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Post-translational Modifications Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.; Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage.
Tissue Specificity Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Swiss-Prot Key P55211_HUMAN
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1
I am interested in this antibody in 0.3mg size, 1mg/mL. Would it be possible to change the storage buffer to PBS and not include sodium azide?
Question by: Guest on 11 Apr 2018 13:40:00
-3
Hello, thank you for your query. If you would like to place a custom order, the product should be at least 1 mg. There will be no additional cost for changing the storage buffer. Please contact us at info@stjohnslabs.com for further information.
Answer by: Customer Support on 11 Apr 2018 13:43:00

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