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Anti-CHOP antibody

[STJ97774] Download PDF Print Data Sheet

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Product name Anti-CHOP antibody
Short Description Mouse Monoclonal to CHOP.
Description CHOP is a protein encoded by the DDIT3 gene which is approximately 19,1 kDa. CHOP is localised to the cytoplasm and nucleus. It is involved in the unfolded protein response, the MAPK signalling pathway and apoptosis modulation and signalling. This protein falls under the CCAAT/enhancer-binding protein family of transcription factors. It functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP and preventing their DNA binding activity. It is activated by endoplasmic reticulum stress and promotes apoptosis. CHOP is expressed in the muscle, nervous system, eye, liver and heart. Mutations in the DDIT3 gene may result in myxoid liposarcoma. STJ97774 was developed from clone 2B1 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody detects endogenous levels of DDIT3
Applications IHC-p, WB
Dilution range WB 1:1000-2000 / IHC 1:100-200
Specificity CHOP Mouse Monoclonal Antibody (2B1) detects endogenous levels of DDIT3
Protein Name DDIT3 - DDIT3 - CHOP10 - GADD153 - CHOP - DNA damage inducible transcript 3
Immunogen synthetic peptide derived from CHOP
Immunogen Region 10-90 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Mouse
Clonality Monoclonal
Clone ID 2B1
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Isotype IgG1
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 1649 (Human)
Gene Symbol DDIT3
Database Links Human UniProt/Swiss-Prot:P35638 Mouse UniPort/Swiss-Prot: P35639 Rat UniProt/Swiss-Port: Q496Y7 Human Entrez Gene: 1649 Mouse Entrez Gene: 13198 Rat Entrez Gene: Rn.11183
Alternative Names DDIT3 / DNA damage inducible transcript 3 / Anapl_18134 antibody / AS27_01521 antibody / CB1_000607009 antibody / CCAAT /enhancer-binding protein homologous protein antibody / CCAAT /enhancer-binding protein-homologous protein antibody / C /EBP homologous protein antibody / C /EBP-homologous protein 10 antibody / C /EBP homoologous protein 10 antibody / C /EBP-like protein antibody / CEBPZ antibody / C /EBP zeta antibody / CHOP antibody / CHOP10 antibody / CHOP-10 antibody / DDIT-3 antibody / DNA damage-inducible transcript 3 antibody / DNA-damage inducible transcript 3 antibody / DNA-damage-inducible transcript 3 antibody / DNA damage-inducible transcript 3 protein antibody / DNA damage-inducible transcript 3 protein-like protein antibody / EGK_03839 antibody / gadd153 antibody / growth arrest and DNA damage-inducible antibody / growth arrest and DNA damage-inducible protein GADD153 antibody / growth arrest and DNA-damage-inducible protein GADD153 antibody / GW7_04685 antibody / H920_14021 antibody / MDA_GLEAN10006654 antibody / N300_09522 antibody / N301_00809 antibody / N335_09969 antibody / PAL_GLEAN10004864 antibody / PANDA_004123 antibody / transcription factor GADD153 antibody / TREES_T100018363 antibody / UY3_00745 antibody / Xchop antibody / Y1Q_008040 antibody / Y956_02569 antibody
Function Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response.
Post-translational Modifications Ubiquitinated, leading to its degradation by the proteasome. ; Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity.
Cellular Localization Cytoplasm. Nucleus. Note=Present in the cytoplasm under non-stressed conditions and ER stress leads to its nuclear accumulation.
Sequence and Domain Family The N-terminal region is necessary for its proteasomal degradation, transcriptional activity and interaction with EP300/P300.

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