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Anti-Cleaved-Caspase-8 p18 (S217) antibody

[STJ97641] Download PDF Print Data Sheet
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Product name Anti-Cleaved-Caspase-8 p18 (S217) antibody
Short Description Rabbit polyclonal to Cleaved-Caspase-8 p18 (S217).
Description Caspase-8 is a protein encoded by the CASP8 gene which is approximately 55,3 kDa. Caspase-8 is localised to the cytoplasm and is involved in the TNFR1 pathway, dimerization of procaspase-8 , activated TLR4 signalling, apoptosis signalling and toll-like receptor signalling pathways. This protein falls under the cysteine-aspartic acid protease family. It pays a role in the programmed cell death induced by Fas and various apoptotic stimuli. Caspase-8 isoform 1, 5 and 7 are expressed in a wide variety of tissues. Mutations in the CASP8 gene may result in a caspase-8 deficiency. STJ97641 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.This polyclonal antibody detects endogenous levels of fragment of activated caspase-8 p18 protein resulting from cleavage adjacent to S217.
Applications ELISA, IF, WB
Dilution range WB 1:500-1:2000
ELISA 1:10000
Specificity Cleaved-Caspase-8 p18 (S217) Polyclonal Antibody detects endogenous levels of Cleaved-Caspase-8 p18 (S217) protein.
Protein Name Caspase-8
CASP-8
Apoptotic cysteine protease
Apoptotic protease Mch-5
CAP4
FADD-homologous ICE/ced-3-like protease
FADD-like ICE
FLICE
ICE-like apoptotic protease 5
MORT1-associated ced-3 homolog
MACH
Caspase-8 subunit p18
Caspase-8 subunit p10
Immunogen Synthesized peptide derived from human Cleaved-Caspase-8 p18 (S217)
Immunogen Region 170-250 aa, Internal
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 841
Gene Symbol CASP8
Database Links HGNC:1509
OMIM:211980
Alternative Names Anti-Cleaved-Caspase-8 p18 (S217) antibody
Function Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Post-translational Modifications Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events. Phosphorylation on Ser-387 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes.
Cellular Localization Cytoplasm.
Tissue Specificity Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
Sequence and Domain Family Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
Swiss-Prot Key Q14790_HUMAN
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I am interested in this antibody in 0.3mg size, 1mg/mL. Would it be possible to change the storage buffer to PBS and not include sodium azide?
Question by: Guest on 11 Apr 2018 13:40:00
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Hello, thank you for your query. If you would like to place a custom order, the product should be at least 1 mg. There will be no additional cost for changing the storage buffer. Please contact us at info@stjohnslabs.com for further information.
Answer by: Customer Support on 11 Apr 2018 13:43:00

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