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Anti-Cleaved-Caspase-8 p18 (S217) antibody

[STJ97641] Download PDF Print Data Sheet

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Product name Anti-Cleaved-Caspase-8 p18 (S217) antibody
Short Description Rabbit Polyclonal to Cleaved-Caspase-8 p18 (S217).
Description Caspase-8 is a protein encoded by the CASP8 gene which is approximately 55,3 kDa. Caspase-8 is localised to the cytoplasm and is involved in the TNFR1 pathway, dimerization of procaspase-8 , activated TLR4 signalling, apoptosis signalling and toll-like receptor signalling pathways. This protein falls under the cysteine-aspartic acid protease family. It pays a role in the programmed cell death induced by Fas and various apoptotic stimuli. Caspase-8 isoform 1, 5 and 7 are expressed in a wide variety of tissues. Mutations in the CASP8 gene may result in a caspase-8 deficiency. STJ97641 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.This polyclonal antibody detects endogenous levels of fragment of activated caspase-8 p18 protein resulting from cleavage adjacent to S217.
Applications ELISA, IF, WB
Dilution range WB 1:500-1:2000 / ELISA 1:10000 /
Specificity Cleaved-Caspase-8 p18 (S217) Polyclonal Antibody detects endogenous levels of Cleaved-Caspase-8 p18 (S217) protein.
Protein Name Caspase-8 - CASP8 - MCH5 - MACH - FLICE - Casp-8 - caspase 8
Immunogen Synthesized peptide derived from human Cleaved-Caspase-8 p18 (S217)
Immunogen Region 170-250 aa, Internal
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 841 (Human) /
Gene Symbol CASP8
Database Links Human UniProt/Swiss-Prot:Q14790 Mouse UniPort/Swiss-Prot: O89110 Rat UniProt/Swiss-Port: Q9JHX4 Human Entrez Gene: 841 Mouse Entrez Gene: 12370 Rat Entrez Gene: Rn.54474
Alternative Names CASP8 / caspase 8 / ALPS2B antibody / Anapl_12750 antibody / apoptotic cysteine protease antibody / apoptotic protease Mch-5 antibody / AS28_03248 antibody / CAP4 antibody / CASP-8 antibody / casp8-A antibody / CASP8-s antibody / Caspase-8 antibody / caspase-8a antibody / caspase 8 / apoptosis-related cysteine peptidase antibody / caspase 8 / apoptosis-related cysteine protease antibody / caspase-8-like cysteine peptidase antibody / CB1_001286010 antibody / cysteine protease antibody / DEATH effector domain caspase antibody / DED caspase antibody / FADD-homologous ICE /CED-3-like protease antibody / FADD-like ICE antibody / Fas-linked ICE-like protease antibody / FLICE antibody / H920_02343 antibody / hypothetical protein antibody / ICE-like apoptotic protease 5 antibody / MACH antibody / MACH-alpha-1 /2 /3 protein antibody / MACH-beta-1 /2 /3 /4 protein antibody / MCH5 antibody / MDA_GLEAN10008508 antibody / MORT1-associated ced-3 homolog antibody / N301_02460 antibody / N302_09791 antibody / N305_06731 antibody / N306_11814 antibody / N310_05621 antibody / N311_03453 antibody / N322_04773 antibody / N328_04229 antibody / N330_10359 antibody / N335_13640 antibody / N336_10212 antibody / N341_03664 antibody / PAL_GLEAN10026114 antibody / PANDA_003926 antibody / TREES_T100003111 antibody / xcaspase 8 antibody / xCaspase-8 antibody / Y1Q_015954 antibody / Y956_07524 antibody / Z169_07017 antibody
Function Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Post-translational Modifications Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events. ; Phosphorylation on Ser-387 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes.
Cellular Localization Cytoplasm.
Tissue Specificity Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
Sequence and Domain Family Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.


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