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Anti-Cleaved-Notch 1 (V1754) antibody

[STJ90066] Download PDF Print Data Sheet

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Product name Anti-Cleaved-Notch 1 (V1754) antibody
Short Description Rabbit Polyclonal to Cleaved-Notch 1 (V1754).
Description Notch 1 is a protein encoded by the NOTCH1 gene which is approximately 272,5 kDa. Notch 1 is localised to the cell membrane. It is involved in the notch signalling pathway and Notch 2 activation and transmission of signal to the nucleus. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Notch 1 is expressed in most adult tissues but is found mainly in lymphoid tissues. Mutations in the NOTCH1 gene may result in aortic valve disease and Adams-Oliver syndrome. STJ90066 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of fragment of activated Notch 1 protein resulting from cleavage adjacent to V1754.
Applications ELISA, WB
Dilution range WB 1:500-1:2000 / IHC 1:100-1:300 / ELISA 1:20000 /
Specificity Cleaved-Notch 1 (V1754) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 1 protein resulting from cleavage adjacent to V1754.
Protein Name Neurogenic locus notch homolog protein 1 - NOTCH1 - TAN1 - notch 1
Immunogen Synthesized peptide derived from human Notch 1
Immunogen Region 1710-1790 aa, Internal
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 4851 (Human) / 18128 (Mouse) /
Gene Symbol NOTCH1
Molecular Weight 110 kDa
Database Links Human UniProt/Swiss-Prot:P46531 Mouse UniPort/Swiss-Prot: Q01705 Rat UniProt/Swiss-Port: Q07008 Human Entrez Gene: 4851 Mouse Entrez Gene: 18128 Rat Entrez Gene: Rn.25046
Alternative Names NOTCH1 / notch 1 / AOS5 antibody / AOVD1 antibody / AS27_13737 antibody / AS28_01090 antibody / Drosophila Notch homolog 1 (controlling the the ectodermal and neural cell fate in Drosophila) antibody / H920_17125 antibody / hN1 antibody / hypothetical protein antibody / I79_019276 antibody / lin-12 antibody / M91_09792 antibody / M959_09342 antibody / major type A protein antibody / Mis6 antibody / Motch A antibody / mT14 antibody / N1 antibody / N300_08786 antibody / N301_12472 antibody / N302_13073 antibody / N303_06196 antibody / N305_12834 antibody / N306_02294 antibody / N307_04845 antibody / N308_09218 antibody / N309_09925 antibody / N310_12413 antibody / N312_03483 antibody / N320_05104 antibody / N321_06684 antibody / N322_04926 antibody / N324_08198 antibody / N325_07685 antibody / N326_00864 antibody / N327_02271 antibody / N328_06489 antibody / N330_08116 antibody / N333_05289 antibody / N334_08523 antibody / N335_09955 antibody / neurogenic locus notch homolog protein 1 antibody / neurogenic locus notch homolog protein 1 preproprotein antibody / Neurogenic locus notch like protein 1 antibody / Neurogenic locus notch-like protein 1 antibody / Neurogenic locus notch protein antibody / Neurogenic locus notch protein 1 antibody / neurogenic locus notch protein 1-like protein antibody / neurogenic locus notch protein homolog antibody / Neurogenic locus notch protein like protein antibody / NOTCH antibody / NOTCH-1 antibody / notch1-a antibody / notch 2 antibody / NOTCH2 antibody / Notch gene homolog 1 antibody / Notch homolog 1 / translocation-associated antibody / p300 antibody / PAL_GLEAN10012684 antibody / TAN1 antibody / translocation-associated notch protein TAN-1 antibody / transmembrane receptor Notch1 antibody / TREES_T100011163 antibody / UY3_08201 antibody / xnotch antibody / xnotch1 antibody / x-notch-1 antibody / Xotch antibody / Y1Q_029491 antibody / Y956_02135 antibody / Z169_10168 antibody
Function Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).
Post-translational Modifications Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by ADAM17 to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). Following endocytosis, this fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. ; Phosphorylated. ; O-glycosylated on the EGF-like domains . Contains both O-linked fucose and O-linked glucose in the EGF-like domains 11, 12 and 13, which are interacting with the residues on DLL4 (By similarity). O-linked glycosylation by GALNT11 is involved in determination of left/right symmetry: glycosylation promotes activation of NOTCH1, possibly by promoting cleavage by ADAM17, modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO) . ; Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH. Monoubiquitination at Lys-1759 is required for activation by gamma-secretase cleavage, it promotes interaction with AAK1, which stabilizes it. Deubiquitination by EIF3F is necessary for nuclear import of activated Notch. ; Hydroxylated at Asn-1955 by HIF1AN. Hydroxylated at Asn-2022 by HIF1AN (By similarity). Hydroxylation reduces affinity for HI1AN and may thus indirectly modulate negative regulation of NICD (By similarity).
Cellular Localization Cell membrane ; Notch 1 intracellular domain: Nucleus Note=Following proteolytical processing NICD is translocated to the nucleus.
Tissue Specificity In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.

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