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Anti-GAPDH antibody

[STJ96931] Download PDF Print Data Sheet

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Product name Anti-GAPDH antibody
Description Mouse Monoclonal to GAPDH.
Host Mouse
Reactivity Cow, Dog, Hamster, Human, Insect, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Yeast
Applications IHC, WB
Immunogen Synthetic Peptide
Gene ID 2597 (Human) / 100042025 (Mouse) / 24383 (Rat)
Dilution range WB 1:5000 / IHC 1:200
Specificity The antibody detects endogenous GAPDH protein.
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Clone ID 2B8
Note For Research Use Only (RUO).
Protein Name Glyceraldehyde-3-phosphate dehydrogenase - GAPDH - GAPD - glyceraldehyde-3-phosphate dehydrogenase
Clonality Monoclonal
Conjugation Unconjugated
Isotype IgG1
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Database Links Human UniProt/Swiss-Prot:P04406 Mouse UniPort/Swiss-Prot: P16858 Rat UniProt/Swiss-Port: P04797 Human Entrez Gene: 2597 Mouse Entrez Gene: 14433 Rat Entrez Gene: Rn.129558
Alternative Names GAPDH / glyceraldehyde-3-phosphate dehydrogenase / 38 kDa BFA-dependent ADP-ribosylation substrate antibody / A306_05224 antibody / aging-associated gene 9 protein antibody / AS27_05473 antibody / AS28_01422 antibody / BARS-38 antibody / bb02e05 antibody / CB1_000216010 antibody / CGI_10010974 antibody / condensin complex subunit 1 antibody / EH28_04863 antibody / epididymis secretory sperm binding protein Li 162eP antibody / G3P antibody / G3PD antibody / G3PDH antibody / gapd antibody / Gapdh_0 antibody / GB14798 antibody / glyceraldehyde 3-phosphate dehydrogenase antibody / glyceraldehyde-3-phosphate dehydrogenase-1 antibody / glyceraldehyde-3-phosphate dehydrogenase (GAPDH) homologue antibody / glyceraldehyde-3-phosphate dehydrogenase-like protein antibody / glyceraldehyde-3-phosphodehydrogenase antibody / glyceraldehyde phosphate dehydrogenase antibody / glyceraldehyde-phosphate dehydrogenase antibody / glyceraldhyde 3-phosphate dehydrogenase antibody / glycerladehyde 3 phosphate dehydrogenase antibody / H920_18369 antibody / HEL-S-162eP antibody / hypothetical protein antibody / I79_001391 antibody / KNC-NDS6 antibody / M959_01780 antibody / MDA_GLEAN10016050 antibody / N300_14152 antibody / N301_07494 antibody / N302_08099 antibody / N303_15805 antibody / N305_07288 antibody / N306_11715 antibody / N307_10662 antibody / N308_11184 antibody / N309_11304 antibody / N310_00356 antibody / N311_07301 antibody / N312_07560 antibody / N320_05893 antibody / N321_12361 antibody / N322_08158 antibody / N324_05388 antibody / N325_08017 antibody / N326_06056 antibody / N327_07230 antibody / N328_01449 antibody / N329_06512 antibody / N330_07693 antibody / N331_12032 antibody / N332_05780 antibody / N333_03254 antibody / N334_08966 antibody / N335_11600 antibody / N336_05030 antibody / N339_03304 antibody / N340_13093 antibody / N341_06027 antibody / PAL_GLEAN10015551 antibody / PANDA_011266 antibody / peptidyl-cysteine S-nitrosylase GAPDH antibody / PsGAPDH antibody / TREES_T100008257 antibody / Y1Q_030375 antibody / Y956_07949 antibody / Z169_15811 antibody
Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Sequence and Domain Family The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
Post-translational Modifications S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus (By similarity). S-nitrosylation of Cys-247 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity. ; ISGylated. ; Sulfhydration at Cys-152 increases catalytic activity. ; Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at Met-46 may play a pivotal role in the formation of these insoluble structures. This modification has been detected in vitro following treatment with free radical donor (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide. It has been proposed to destabilize nearby residues, increasing the likelihood of secondary oxidative damages, including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and aggregation.
Cellular Localization Cytoplasm, cytosol Nucleus Cytoplasm, perinuclear region Membrane Cytoplasm, cytoskeleton Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.

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