Anti-GAPDH antibody

[STJ96931] Download PDF Print Data Sheet

Western blot (WB) analysis of Hela, rat brain, rabbit muscle, sheep muscle and mouse brain cells, using monoclonal Anti-GAPDH Antibody (STJ96931) at 1:10000.

Immunohistochemical analysis of human-colon tissue. Anti-GAPDH at 1:200 (4°C,overnight). Antigen retrieval - Sodium Citrate pH6 (>98°C, 20min). Secondary - 1:200 (room temp, 30min). Negative control - Secondary only

Immunohistochemical analysis of human stomach tissue. Anti-GAPDH at 1:200 (4°C,overnight). Antigen retrieval - Sodium Citrate pH6 (>98°C, 20min). Secondary - 1:200 (room temp, 30min). Negative control - Secondary only

Immunohistochemical analysis of mouse heart tissue. Anti-GAPDH at 1:200 (4°C,overnight). Antigen retrieval - Sodium Citrate pH6 (>98°C, 20min). Secondary - 1:200 (room temp, 30min). Negative control - Secondary only

Immunohistochemical analysis of mouse kidney tissue. Anti-GAPDH at 1:200 (4°C,overnight). Antigen retrieval - Sodium Citrate pH6 (>98°C, 20min). Secondary - 1:200 (room temp, 30min). Negative control - Secondary only

Immunohistochemical analysis of rat heart tissue. Anti-GAPDH at 1:200 (4°C,overnight). Antigen retrieval - Sodium Citrate pH6 (>98°C, 20min). Secondary - 1:200 (room temp, 30min). Negative control - Secondary only

Immunohistochemical analysis of rat kidney tissue. Anti-GAPDH at 1:200 (4°C,overnight). Antigen retrieval - Sodium Citrate pH6 (>98°C, 20min). Secondary - 1:200 (room temp, 30min). Negative control - Secondary only

Lead 5-7 days

100μl £150.00
200μl £180.00
50μl £99.00

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General Information
Product Properties
Target

Product name	Anti-GAPDH antibody
Short Description	Mouse monoclonal to GAPDH.
Description	GAPDH is a protein encoded by the GAPDH gene which is approximately 36 kDa. GAPDH is localised to the cytoplasm and nucleus. It is involved in glucose metabolism, respiratory electron transport, carbon metabolism and HIF-1 signalling pathway. It is a moonlighting protein based on its ability to perform mechanistically distinct functions. It catalyses an important energy-yielding step in carbohydrate metabolism and also has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions. GAPDH is expressed in the blood, eyes, intestine, kidney and liver. Mutations in the GAPDH gene may result in FMR1-related disorders. STJ96931 was developed from clone 2B8 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody detects endogenous GAPDH protein.
Applications	IHC-p, WB
Dilution range	WB 1:5000 IHC 1:200
Specificity	The antibody detects endogenous GAPDH protein.
Protein Name	Glyceraldehyde-3-phosphate dehydrogenase GAPDH Peptidyl-cysteine S-nitrosylase GAPDH
Immunogen	Synthetic Peptide
Storage Instruction	Store at -20°C, and avoid repeat freeze-thaw cycles.
Note	For Research Use Only (RUO).

Host	Mouse
Clonality	Monoclonal
Clone ID	2B8
Reactivity	Bovine, Dog, Hamster, Insect, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Yeast, Human
Conjugation	Unconjugated
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Isotype	IgG1
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Gene ID	2597
Gene Symbol	GAPDH
Database Links	HGNC:4141 OMIM:138400
Alternative Names	Anti-GAPDH antibody
Function	Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules . Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Post-translational Modifications	S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus . S-nitrosylation of Cys-247 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity. ISGylated. Sulfhydration at Cys-152 increases catalytic activity. Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at Met-46 may play a pivotal role in the formation of these insoluble structures. This modification has been detected in vitro following treatment with free radical donor (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide. It has been proposed to destabilize nearby residues, increasing the likelihood of secondary oxidative damages, including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and aggregation.
Cellular Localization	Cytoplasm, cytosol Nucleus Cytoplasm, perinuclear region Membrane Cytoplasm, cytoskeleton. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal . Postnuclear and Perinuclear regions.
Sequence and Domain Family	The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
Swiss-Prot Key	P04406_HUMAN

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I am interested in this antibody in 0.3mg size, 1mg/mL. Would it be possible to change the storage buffer to PBS and not include sodium azide?

Question by: Guest on 11 Apr 2018 13:40:00

-4

Hello, thank you for your query. If you would like to place a custom order, the product should be at least 1 mg. There will be no additional cost for changing the storage buffer. Please contact us at info@stjohnslabs.com for further information.

Answer by: Customer Support on 11 Apr 2018 13:43:00

-4

Answer by: Customer Support on 11 Apr 2018 13:43:00

Anti-GAPDH antibody

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