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Anti-Glucocorticoid receptor antibody

[STJ97101] Download PDF Print Data Sheet

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Product name Anti-Glucocorticoid receptor antibody
Short Description Rabbit Polyclonal to Glucocorticoid receptor.
Description Glucocorticoid receptor is a protein encoded by the NR3C1 gene which is approximately 85,6 kDa. The glucocorticoid receptor is localised to the cytoplasm, mitochondrion and nucleus. It is involved in PEDF induced signalling, gene expression and the nuclear receptor transcription pathway. It is a protein which can function both as a transcription factor that binds to glucocorticoid response elements to activate their transcription, and as a regulator of other transcription factors. The glucocorticoid receptor is widely expressed. Mutations in the NR3C1 gene may result in glucocorticoid resistance. STJ97101 was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. This antibody detects endogenous GR proteins.
Applications WB
Dilution range WB 1:1000-3000
Specificity The antibody detects endogenous GR proteins.
Protein Name Glucocorticoid receptor - NR3C1 - GR - GRL - nuclear receptor subfamily 3 group C member 1
Immunogen Recombinant Protein
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 2908 (Human) / 24413 (Rat)
Gene Symbol NR3C1
Database Links Human UniProt/Swiss-Prot:P04150 Rat UniProt/Swiss-Port: P06536 Human Entrez Gene: 2908 Mouse Entrez Gene: 14815 Rat Entrez Gene: Rn.90070
Alternative Names NR3C1 / nuclear receptor subfamily 3 group C member 1 / A306_12420 antibody / Anapl_07313 antibody / AS27_12115 antibody / AS28_00691 antibody / CB1_000740057 antibody / fb13f09 antibody / GCCR antibody / GCL antibody / Gcr antibody / GCRST antibody / glucocorticoid nuclear receptor variant 1 antibody / Glucocorticoid receptor antibody / glucocorticoid receptor 1 antibody / glucocorticoid receptor 2b antibody / glucocorticoid receptor alpha antibody / glucocorticoid receptor GR1a antibody / glucocorticoid receptor GR1b antibody / glucocorticoid receptor isoform alpha antibody / glucocorticoid receptor isoform alpha variant 1 antibody / glucocorticoid receptor isoform alpha variant 2 antibody / glucocorticoid receptor variant 1A antibody / glucocorticoid receptor variant 1B antibody / glucocorticoid receptor variant 1C antibody / glucocorticoid receptor variant 1D antibody / glucocorticoid receptor variant 1E antibody / glucocorticoid receptor variant 1F antibody / glucocorticoid receptor variant 1G antibody / glucocorticoid receptor variant 1H antibody / glucocorticoid receptor variant 1H2 antibody / glucocorticoid receptor variant 1J antibody / glucocorticoid receptor variant beta antibody / glucocorticoid receptor variant gamma antibody / glucocorticoid receptor variant P antibody / gr antibody / gr1 antibody / gr2b antibody / GR-A antibody / GRL antibody / Grl1 antibody / Grl-1 antibody / GW7_10599 antibody / H920_06531 antibody / hypothetical protein antibody / I79_008992 antibody / M91_20487 antibody / M959_03929 antibody / N300_11000 antibody / N302_05397 antibody / N305_06806 antibody / N306_02138 antibody / N307_00733 antibody / N308_05274 antibody / N309_08418 antibody / N310_02849 antibody / N312_01082 antibody / N320_11508 antibody / N321_06584 antibody / N322_03428 antibody / N325_01920 antibody / N327_02652 antibody / N328_06291 antibody / N329_04059 antibody / N330_14144 antibody / N335_11507 antibody / N339_03930 antibody / N340_05656 antibody / N341_03928 antibody / NO antibody / nuclear receptor subfamily 3 / group C / member 1 antibody / nuclear receptor subfamily 3 / group C / member 1 (glucocorticoid receptor) antibody / PAL_GLEAN10016706 antibody / PANDA_000333 antibody / TREES_T100021452 antibody / utouto antibody / utut antibody / UY3_10605 antibody / xGR antibody / Y1Q_005888 antibody / Y956_06273 antibody
Function Receptor for glucocorticoids (GC) . Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling . Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay . Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity). ; Isoform Alpha: Has transcriptional activation and repression activity . Mediates glucocorticoid-induced apoptosis . Promotes accurate chromosome segregation during mitosis . May act as a tumor suppressor . May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity). ; Isoform Beta: Acts as a dominant negative inhibitor of isoform Alpha . Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed . Loses this transcription modulator function on its own . Has no hormone-binding activity . May play a role in controlling glucose metabolism by maintaining insulin sensitivity (By similarity). Reduces hepatic gluconeogenesis through down-regulation of PEPCK in an isoform Alpha-dependent manner . Directly regulates STAT1 expression in isoform Alpha-independent manner . ; Isoform Alpha-2: Has lower transcriptional activation activity than isoform Alpha. Exerts a dominant negative effect on isoform Alpha trans-repression mechanism .; Isoform GR-P: Increases activity of isoform Alpha. ; Isoform Alpha-B: More effective than isoform Alpha in transcriptional activation, but not repression activity. ; Isoform 10: Has transcriptional activation activity. ; Isoform Alpha-C1: Has transcriptional activation activity. ; Isoform Alpha-C2: Has transcriptional activation activity. ; Isoform Alpha-C3: Has highest transcriptional activation activity of all isoforms created by alternative initiation . Has transcriptional repression activity . Mediates glucocorticoid-induced apoptosis . ; Isoform Alpha-D1: Has transcriptional activation activity. ; Isoform Alpha-D2: Has transcriptional activation activity. ; Isoform Alpha-D3: Has lowest transcriptional activation activity of all isoforms created by alternative initiation . Has transcriptional repression activity .
Post-translational Modifications Acetylation by CLOCK reduces its binding to glucocorticoid response elements and its transcriptional activity. ; Increased proteasome-mediated degradation in response to glucocorticoids . Isoform Alpha-B appears to be more susceptible to proteolytic degradation than isoform Alpha . ; Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203, Ser-226 and Ser-404-phosphorylated forms are mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear . Phosphorylation at Ser-211 increases transcriptional activity . Phosphorylation at Ser-203, Ser-226 and Ser-404 decreases signaling capacity . Phosphorylation at Ser-404 may protect from glucocorticoid-induced apoptosis . Phosphorylation at Ser-203 and Ser-211 is not required in regulation of chromosome segregation . May be dephosphorylated by PPP5C, attenuates NR3C1 action (By similarity). ; Sumoylation at Lys-277 and Lys-293 negatively regulates its transcriptional activity . Sumoylation at Lys-703 positively regulates its transcriptional activity in the presence of RWDD3 (By similarity). Sumoylation at Lys-277 and Lys-293 is dispensable whereas sumoylation at Lys-703 is critical for the stimulatory effect of RWDD3 on its transcriptional activity (By similarity). Heat shock increases sumoylation in a RWDD3-dependent manner (By similarity). ; Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
Cellular Localization Isoform Alpha: Cytoplasm Nucleus Mitochondrion Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=After ligand activation, translocates from the cytoplasm to the nucleus. ; Isoform Beta: Nucleus Cytoplasm Note=Expressed predominantly in the nucleus with some expression also detected in the cytoplasm. ; Isoform Alpha-B: Nucleus Cytoplasm Note=After ligand activation, translocates from the cytoplasm to the nucleus.
Tissue Specificity Widely expressed including bone, stomach, lung, liver, colon, breast, ovary, pancreas and kidney . In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole
Sequence and Domain Family Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain . The ligand-binding domain is required for correct chromosome segregation during mitosis although ligand binding is not required .

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