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Anti-GSK3β antibody

[STJ93447] Download PDF Print Data Sheet

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Product name Anti-GSK3β antibody
Short Description Rabbit Polyclonal to GSK3β.
Description GSK3β is a protein encoded by the GSK3B gene which is approximately 46,7 kDa. GSK3β is localised to the cytoplasm, nucleus and cell membrane. It is involved in common cytokine receptor gamma-chain family signalling pathways, RET signalling, regulation of lipid metabolism and insulin signalling-generic cascades. It is a constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signalling and regulation of transcription factors and microtubules. In skeletal muscle, it contributes to insulin regulation of glycogen synthesis. GSK3β is expressed in the testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Mutations in the GSK3B gene may result in Alzheimer disease and Usher syndrome. STJ93447 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of GSK3β protein.
Applications IHC-p, ELISA, IP, WB
Dilution range WB 1:500-1:2000 / IHC 1:100-1:300 / IP 1:200-500 / ELISA 1:20000 /
Specificity GSK3β Polyclonal Antibody detects endogenous levels of GSK3β protein.
Protein Name Glycogen synthase kinase-3 beta - GSK3B - glycogen synthase kinase 3 beta
Immunogen Synthesized peptide derived from human GSK3β around the non-phosphorylation site of S9.
Immunogen Region 1-80 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 2932 (Human) / 56637 (Mouse) / 84027 (Rat)
Gene Symbol GSK3B
Molecular Weight 47 kDa
Database Links Human UniProt/Swiss-Prot:P49841 Mouse UniPort/Swiss-Prot: Q9WV60 Rat UniProt/Swiss-Port: P18266 Human Entrez Gene: 2932 Mouse Entrez Gene: 56637 Rat Entrez Gene: Rn.10426
Alternative Names GSK3B / glycogen synthase kinase 3 beta / A306_07223 antibody / Anapl_13534 antibody / AS27_09821 antibody / AS28_01467 antibody / C86142 antibody / CB1_001373021 antibody / D623_10031791 antibody / FA antibody / factor A antibody / fb68h05 antibody / fk80d11 antibody / Glycogen synthase kinase-3 beta antibody / glycogen synthase kinase-3 beta-like antibody / glycogen synthase kinase-3 beta-like protein antibody / glycogen synthase kinase-3 beta splice variant X1 antibody / glycogen synthase kinase-3 beta splice variant X2 antibody / glycogen synthase kinase-3 beta splice variant X3 antibody / glycogen synthase kinase 3 beta variant 1 antibody / glycogen synthase kinase-3 beta variant 1 antibody / glycogen synthase kinase 3 beta variant 2 antibody / glycogen synthase kinase-3 beta variant 2 antibody / glycogen synthase kinase 3 beta variant 3 antibody / glycogen synthase kinase-3 beta variant 3 antibody / glycogen synthase kinase 3 beta variant 4 antibody / glycogen synthase kinase-3 beta variant 4 antibody / glycogen synthase kinase 3 beta variant 5 antibody / glycogen synthase kinase-3 beta variant 5 antibody / glycogen synthase kinase-3 beta variant 6 antibody / gsk3 antibody / GSK-3 antibody / GSK-3[b] antibody / GSK3B1 antibody / GSK3b2 antibody / GSK3B3 antibody / GSK3B4 antibody / GSK3B5 antibody / GSK3B6 antibody / gsk3-beta antibody / GSK3beta antibody / GSK-3beta antibody / GSK-3 beta antibody / GSK3beta isoform antibody / hypothetical protein antibody / intracellular kinase antibody / M91_01371 antibody / M959_12429 antibody / MDA_GLEAN10018598 antibody / N300_13586 antibody / N301_03177 antibody / N302_08006 antibody / N303_06935 antibody / N306_10658 antibody / N307_11393 antibody / N308_00063 antibody / N311_03429 antibody / N312_01936 antibody / N322_11442 antibody / N325_04935 antibody / N329_02185 antibody / N335_05899 antibody / N339_00758 antibody / PANDA_003271 antibody / serine /threonine-protein kinase GSK3B antibody / TREES_T100015347 antibody / xgsk-3 antibody / Y1Q_030349 antibody / Y956_11541 antibody / Z169_15769 antibody
Function Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin. Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules. MAPT/TAU is the principal component of neurofibrillary tangles in Alzheimer disease. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA). Negatively regulates replication in pancreatic beta-cells, resulting in apoptosis, loss of beta-cells and diabetes. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation. Phosphorylates MUC1 in breast cancer cells, decreasing the interaction of MUC1 with CTNNB1/beta-catenin. Is necessary for the establishment of neuronal polarity and axon outgrowth. Phosphorylates MARK2, leading to inhibit its activity. Phosphorylates SIK1 at 'Thr-182', leading to sustain its activity. Phosphorylates ZC3HAV1 which enhances its antiviral activity. Phosphorylates SNAI1, leading to its BTRC-triggered ubiquitination and proteasomal degradation. Phosphorylates SFPQ at 'Thr-687' upon T-cell activation. Phosphorylates NR1D1 st 'Ser-55' and 'Ser-59' and stabilizes it by protecting it from proteasomal degradation. Regulates the circadian clock via phosphorylation of the major clock components including ARNTL/BMAL1, CLOCK and PER2. Phosphorylates CLOCK AT 'Ser-427' and targets it for proteasomal degradation. Phosphorylates ARNTL/BMAL1 at 'Ser-17' and 'Ser-21' and primes it for ubiquitination and proteasomal degradation. Phosphorylates OGT at 'Ser-3' or 'Ser-4' which positively regulates its activity. Phosphorylates MYCN in neuroblastoma cells which may promote its degradation .
Post-translational Modifications Phosphorylated by AKT1 and ILK1. Upon insulin-mediated signaling, the activated PKB/AKT1 protein kinase phosphorylates and desactivates GSK3B, resulting in the dephosphorylation and activation of GYS1. Activated by phosphorylation at Tyr-216 . Inactivated by phosphorylation at Ser-9 (Probable). ; Mono-ADP-ribosylation by PARP10 negatively regulates kinase activity.
Cellular Localization Cytoplasm Nucleus. Cell membrane. Note=The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane.
Tissue Specificity Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain.

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