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Anti-MARK1/2/3/4 antibody

[STJ94015] Download PDF Print Data Sheet

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Product name Anti-MARK1/2/3/4 antibody
Short Description Rabbit Polyclonal to MARK1/2/3/4.
Description MARK1/2/3/4 is a protein encoded by the MARK1 gene which is approximately 89 kDa. MARK1/2/3/4 is localised to the cell membrane and cytoplasm. It is involved in cytoplasm signalling and glucose/energy metabolism. It plays a role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. MARK1/2/3/4 is highly expressed in the heart, skeletal muscle, brain and foetal kidney. Mutations in the MARK1 gene may result in Alzheimer disease. STJ94015 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of MARK1/2/3/4 protein.
Applications IHC-p, ELISA, IF, WB
Dilution range WB 1:500-1:2000 / IHC 1:100-1:300 / IF 1:200-1:1000 / ELISA 1:10000 /
Specificity MARK1/2/3/4 Polyclonal Antibody detects endogenous levels of MARK1/2/3/4 protein.
Protein Name Serine/threonine-protein kinase MARK1/2/3/4 - MARK1 - MARK - PAR-1C - microtubule affinity regulating kinase 1
Immunogen Synthesized peptide derived from human MARK1/2/3/4 around the non-phosphorylation site of T215.
Immunogen Region 150-230 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Monkey, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 4139/2011/4140/57787 (Human) / 226778/13728/17169/232944 (Mouse) / 117016/60328/170577 (Rat)
Gene Symbol MARK1
Molecular Weight 89 kDa
Database Links Human UniProt/Swiss-Prot:Q9P0L2 Human Entrez Gene: 4139
Alternative Names MARK1 / microtubule affinity regulating kinase 1 / A306_11311 antibody / AS27_10783 antibody / AS28_00165 antibody / CB1_001073065 antibody / chemokine CCL-CUc antibody / EH28_10486 antibody / ELKL motif serine-threonine protein kinase 3 antibody / ELKL motif serine /threonine-protein kinase 3 antibody / Emk3 antibody / H920_13153 antibody / I79_000079 antibody / M959_04508 antibody / MAP /microtubule affinity regulating kinase 1 antibody / MAP /microtubule affinity-regulating kinase 1 antibody / MAP /microtubule affinity-regulating kinase 3 isoformX1 antibody / MARK antibody / MDA_GLEAN10022862 antibody / mPar-1c antibody / N300_14724 antibody / N301_06433 antibody / N302_00742 antibody / N303_05332 antibody / N305_12771 antibody / N306_11584 antibody / N307_01822 antibody / N308_02484 antibody / N309_10726 antibody / N310_05109 antibody / N311_09473 antibody / N312_02085 antibody / N320_02317 antibody / N321_12235 antibody / N322_05199 antibody / N325_06969 antibody / N326_06372 antibody / N327_01536 antibody / N328_06285 antibody / N329_07315 antibody / N330_03558 antibody / N331_03290 antibody / N332_14229 antibody / N333_00681 antibody / N334_07376 antibody / N335_02976 antibody / N336_08458 antibody / N339_04097 antibody / N340_01457 antibody / N341_08813 antibody / PAL_GLEAN10012200 antibody / PANDA_008740 antibody / Par1c antibody / Par-1c antibody / PAR1 homolog c antibody / Serine /threonine-protein kinase MARK1 antibody / serine /threonine-protein kinase MARK1-like antibody / UY3_11678 antibody / Y1Q_000011 antibody / Y956_07707 antibody / Z169_01533 antibody
Function Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2, MAP4 and MAPT/TAU. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).
Post-translational Modifications Phosphorylation at Thr-613 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity (By similarity). Phosphorylated at Thr-215 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-215 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-219 by GSK3-beta (GSK3B) inhibits the kinase activity.
Cellular Localization Cell membrane Cytoplasm, cytoskeleton Note=Appears to localize to an intracellular network.
Tissue Specificity Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney.
Sequence and Domain Family The UBA domain does not seem to bind ubiquitin and ubiquitin-like and might play a role in regulating the enzyme conformation and localization. Activation of the kinase activity following phosphorylation at Thr-208 is accompanied by a conformational change that alters the orientation of the UBA domain with respect to the catalytic domain (By similarity). ; The KA1 domain mediates binding to phospholipids and targeting to membranes. Binds phosphatidic acid (PA), phosphatidylserine (PtdSer) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2).

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