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Anti-mTOR antibody

[STJ94279] Download PDF Print Data Sheet

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Product name Anti-mTOR antibody
Short Description Rabbit Polyclonal to mTOR.
Description mTOR is a protein encoded by the MTOR gene which is approximately 288,9 kDa. mTOR is localised to the endoplasmic reticulum membrane and mitochondrion outer membrane. It is involved in RET signalling, regulation of lipid metabolism, mTOR signalling and glioma. This protein falls under the phosphatidylinositol kinase family. It is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. mTOR is expressed in numerous tissues, with highest levels expressed in the testis. Mutations in the MTOR gene may result in Smith-Kingsmore syndrome. STJ94279 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of mTOR protein.
Applications IHC-p, ELISA, WB
Dilution range WB 1:500-1:2000 / IHC 1:100-1:300 / ELISA 1:10000 /
Specificity mTOR Polyclonal Antibody detects endogenous levels of mTOR protein.
Protein Name Serine/threonine-protein kinase mTOR - MTOR - RAFT1 - RAPT1 - FLJ44809 - FRAP - FRAP2 - FRAP1 - mechanistic target of rapamycin
Immunogen Synthesized peptide derived from human mTOR around the non-phosphorylation site of T2446.
Immunogen Region 2390-2470 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 2475 (Human) / 56717 (Mouse) / 56718 (Rat)
Gene Symbol MTOR
Molecular Weight 220 kDa
Database Links Human UniProt/Swiss-Prot:P42345 Mouse UniPort/Swiss-Prot: Q9JLN9 Rat UniProt/Swiss-Port: P42346 Human Entrez Gene: 2475 Mouse Entrez Gene: 56717 Rat Entrez Gene: Rn.11008
Alternative Names MTOR / mechanistic target of rapamycin / angiopoietin-like factor CDT6 antibody / AS27_12991 antibody / Bx34 antibody / CB1_000455021 antibody / CG8274-PA antibody / CG8274-PB antibody / D623_10022009 antibody / Dmel_CG8274 antibody / FK506 binding protein 12-rapamycin associated protein 1 antibody / FK506 binding protein 12-rapamycin associated protein 2 antibody / FK506-binding protein 12-rapamycin complex-associated protein 1 antibody / FKBP12-rapamycin complex-associated protein antibody / FKBP12-rapamycin complex-associated protein 1 antibody / FKBP-rapamycin associated protein antibody / FKBP-rapamycin associated protein (FRAP) antibody / FKBP-rapamycin-associated protein FRAP antibody / flat antibody / FRAP antibody / Frap1 antibody / FRAP2 antibody / GB11086 antibody / H920_06981 antibody / hypothetical protein antibody / I79_022096 antibody / lethal (2) k03905 antibody / M959_03308 antibody / mammalian target of rapamycin antibody / MDA_GLEAN10024380 antibody / mechanistic target of rapamycin (serine /threonine kinase) antibody / megator antibody / Mtor-PA antibody / Mtor-PB antibody / N301_11351 antibody / N302_14838 antibody / N303_02585 antibody / N306_05329 antibody / N307_01442 antibody / N308_08301 antibody / N309_08359 antibody / N311_10472 antibody / N312_07948 antibody / N325_05609 antibody / N327_08207 antibody / N329_05123 antibody / N332_00568 antibody / N333_01433 antibody / N336_10311 antibody / N339_00887 antibody / N340_00864 antibody / N341_11411 antibody / PAL_GLEAN10013002 antibody / PANDA_011807 antibody / RAFT1 antibody / rapamycin and FKBP12 target 1 antibody / rapamycin and FKBP12 target-1 protein antibody / rapamycin associated protein FRAP2 antibody / rapamycin target protein 1 antibody / rapt1 antibody / serine /threonine kinase antibody / Serine /threonine-protein kinase mTOR antibody / SKS antibody / target of rapamycin antibody / tor antibody / TPR antibody / Y1Q_007821 antibody / Y956_10736 antibody / Z169_14410 antibody
Function Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1. Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP. mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor. Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules. As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton. Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B. mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-422'. Regulates osteoclastogensis by adjusting the expression of CEBPB isoforms (By similarity).
Post-translational Modifications Autophosphorylates when part of mTORC1 or mTORC2. Phosphorylation at Ser-1261, Ser-2159 and Thr-2164 promotes autophosphorylation. Phosphorylation in the kinase domain modulates the interactions of MTOR with RPTOR and PRAS40 and leads to increased intrinsic mTORC1 kinase activity. Phosphorylation at Thr-2173 in the ATP-binding region by AKT1 strongly reduces kinase activity.
Cellular Localization Endoplasmic reticulum membrane Golgi apparatus membrane Mitochondrion outer membrane Lysosome Cytoplasm Nucleus, PML body Microsome membrane Note=Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB .
Tissue Specificity Expressed in numerous tissues, with highest levels in testis.
Sequence and Domain Family The kinase domain (PI3K/PI4K) is intrinsically active but has a highly restricted catalytic center. ; The FAT domain forms three discontinuous subdomains of alpha-helical TPR repeats plus a single subdomain of HEAT repeats. The four domains pack sequentially to form a C-shaped a-solenoid that clamps onto the kinase domain .

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