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Anti-p21 antibody

[STJ97470] Download PDF Print Data Sheet
5-7 days

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Product name Anti-p21 antibody
Short Description Rabbit polyclonal to p21.
Description p21 is a protein encoded by the CDKN1A gene which is approximately 18,1 kDa. p21 is localised to the cytoplasm and nucleus. It is involved in CDK-mediated phosphorylation and removal of Cdc6, RET signalling, GAB1 signalosome and cell cycle regulation. This protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and functions as a regulator of cell cycle progression at G1. p21 is expressed in all adult tissues. Mutations in the CDKN1A gene may result in pancreatic cancer and ovarian cancer. STJ97470 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody detects endogenous p21 protein.
Applications IHC-p, WB
Dilution range WB 1:1000-2000
IHC 1:100-200
Specificity The antibody detects endogenous p21 protein.
Protein Name Cyclin-dependent kinase inhibitor 1
CDK-interacting protein 1
Melanoma differentiation-associated protein 6
MDA-6
p21
Immunogen Synthesized peptide derived from human p21 around the non-phosphorylation site of T145.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Mouse, Rat, Human
Conjugation Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 1026
Gene Symbol CDKN1A
Database Links HGNC:1784
OMIM:116899
Alternative Names Anti-p21 antibody
Function May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding .
Post-translational Modifications Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. UV radiation-induced phosphorylation at Thr-80 by LKB1 and at Ser-146 by NUAK1 leads to its degradation. Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Ubiquitination at Ser-2 leads to degradation by the proteasome pathway. Ubiquitinated by RNF114; leading to proteasomal degradation. Acetylation leads to protein stability. Acetylated in vitro on Lys-141, Lys-154, Lys-161 and Lys-163. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1 .
Cellular Localization Cytoplasm. Nucleus.
Tissue Specificity Expressed in all adult tissues, with 5-fold lower levels observed in the brain.
Sequence and Domain Family The PIP-box K+4 motif mediates both the interaction with PCNA and the recruitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.; The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex.
Swiss-Prot Key P38936_HUMAN
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