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Anti-PCNA antibody

[STJ96933] Download PDF Print Data Sheet

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Product name Anti-PCNA antibody
Short Description Mouse Monoclonal to PCNA.
Description PCNA is a protein encoded by the PCNA gene which is approximately 28,7 kDa. PCNA is localised to the nucleus. It is involved in the telomere C-strand synthesis and trans-lesion synthesis. It is found in the nucleus and is a cofactor of DNA polymerase-delta. It acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is expressed in the liver, lung, bone marrow, muscle and kidney. Mutations in the PCNA gene may result in ataxia-telangiectasia-like disorder. STJ96933 was developed from clone 12D10 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous PCNA protein.
Applications IHC-p, WB
Dilution range WB 1:5000 / IHC 1:200
Specificity The antibody detects endogenous PCNA protein.
Protein Name Proliferating cell nuclear antigen - PCNA - proliferating cell nuclear antigen
Immunogen Synthetic Peptide
Immunogen Region N-term
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Mouse
Clonality Monoclonal
Clone ID 12D10
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Isotype IgG1
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 5111 (Human) / 18538 (Mouse) / 25737 (Rat)
Gene Symbol PCNA
Database Links Human UniProt/Swiss-Prot:P12004 Mouse UniPort/Swiss-Prot: P17918 Rat UniProt/Swiss-Port: P04961 Human Entrez Gene: 5111 Mouse Entrez Gene: 18538 Rat Entrez Gene: Rn.223
Alternative Names PCNA / proliferating cell nuclear antigen / 53 /13 antibody / A306_04088 antibody / AS27_01336 antibody / AS28_04342 antibody / ATLD2 antibody / CB1_000951033 antibody / CG9193-PA antibody / CG9193-PB antibody / cyclin antibody / Dmel_CG9193 antibody / DmPCNA antibody / DNA polymerase delta auxiliary protein antibody / dPCNA antibody / EH28_15625 antibody / etID36690.10 antibody / fa28e03 antibody / fb36g03 antibody / GW7_03228 antibody / H920_16428 antibody / M959_11405 antibody / MDA_GLEAN10025036 antibody / mus209 antibody / mus 209 antibody / mutagen-sensitive 209 antibody / N300_15807 antibody / N301_02361 antibody / N302_02047 antibody / N303_13161 antibody / N305_06038 antibody / N306_11993 antibody / N307_13234 antibody / N308_00190 antibody / N309_03449 antibody / N311_05952 antibody / N312_07447 antibody / N320_05164 antibody / N321_00934 antibody / N322_01871 antibody / N324_10756 antibody / N325_10799 antibody / N326_01624 antibody / N327_11589 antibody / N328_05887 antibody / N329_09794 antibody / N330_11149 antibody / N331_07552 antibody / N332_11902 antibody / N333_04753 antibody / N334_09468 antibody / N335_12266 antibody / N336_05421 antibody / N339_09700 antibody / N340_06008 antibody / N341_09937 antibody / PAL_GLEAN10024098 antibody / PANDA_014275 antibody / pcna-A antibody / Pcna /cyclin antibody / PCNA /mus209 antibody / PCNA-PA antibody / PCNA-PB antibody / PCNAR antibody / proliferating-cell nuclear antigen antibody / Proliferating-cell-nuclear-antigen antibody / proliferating cell nuclear antigen-like protein antibody / proliferating cellular nuclear antigen antibody / putative proliferating cell nuclear antigen antibody / putative proliferating cell nuclear antigen variant 1 antibody / putative proliferating cell nuclear antigen variant 2 antibody / TREES_T100018085 antibody / UY3_12361 antibody / Y1Q_014204 antibody / Z169_05233 antibody
Function Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways . Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Post-translational Modifications Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA. ; Acetylated by CREBBP and p300/EP300; preferentially acetylated by CREBBP on Lys-80, Lys-13 and Lys-14 and on Lys-77 by p300/EP300 upon loading on chromatin in response to UV irradiation . Lysine acetylation disrupts association with chromatin, hence promoting PCNA ubiquitination and proteasomal degradation in response to UV damage in a CREBBP- and EP300-dependent manner . Acetylation disrupts interaction with NUDT15 and promotes degradation . ; Ubiquitinated . Following DNA damage, can be either monoubiquitinated to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitinated to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms. Following induction of replication stress, monoubiquitinated by the UBE2B-RAD18 complex on Lys-164, leading to recruit translesion (TLS) polymerases, which are able to synthesize across DNA lesions in a potentially error-prone manner. An error-free pathway also exists and requires non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH. This error-free pathway, also known as template switching, employs recombination mechanisms to synthesize across the lesion, using as a template the undamaged, newly synthesized strand of the sister chromatid. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis. Sumoylated during S phase. ; Methylated on glutamate residues by ARMT1/C6orf211.
Cellular Localization Nucleus Note=Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage . Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.


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