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Anti-Phospho-c-Abl (Y245) antibody

[STJ90694] Download PDF Print Data Sheet
Anti-Phospho-c-Abl (Y245) antibody

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Product name Anti-Phospho-c-Abl (Y245) antibody
Short Description Rabbit Polyclonal to Phospho-c-Abl (Y245).
Description c-Abl is a protein encoded by the ABL1 gene which is approximately 122,8 kDa. c-Abl is localised to the cytoplasm, nucleus and mitochondrion. It is involved in DNA double-strand break repair, development Slit-Robo signalling, cell cycle and the ErbB signalling pathway. It coordinates actin re-modelling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like branch formation, membrane anchoring, signalling and microtubule-binding proteins. c-Abl is widely expressed in human tissues. Mutations in the ABL1 gene result in chronic myeloid leukemia. STJ90694 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous c-Abl protein which only binds about Y245 when Y245 is phosphorylated.
Applications ELISA, IF, WB
Dilution range WB 1:500-1:2000 / IF 1:200-1:1000 / ELISA 1:5000 /
Specificity Phospho-c-Abl (Y245) Polyclonal Antibody detects endogenous levels of c-Abl protein only when phosphorylated at Y245.
Protein Name Tyrosine-protein kinase ABL1 - ABL1 - JTK7 - c-ABL - p150 - ABL - ABL proto-oncogene 1 - non-receptor tyrosine kinase
Immunogen Synthesized peptide derived from human c-Abl around the phosphorylation site of Y245.
Immunogen Region 170-250 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 25 (Human) / 11350 (Mouse) /
Gene Symbol ABL1
Molecular Weight 120 kDa
Database Links Human UniProt/Swiss-Prot:P00519 Mouse UniPort/Swiss-Prot: P00520 Rat UniProt/Swiss-Port: E9PT20 Human Entrez Gene: 25 Mouse Entrez Gene: 11350 Rat Entrez Gene: Rn.3105
Alternative Names ABL1 / ABL proto-oncogene 1 / non-receptor tyrosine kinase / Abelson murine leukemia oncogene antibody / Abelson murine leukemia viral oncogene 1-like protein antibody / abelson murine leukemia viral oncogene homolog 1 antibody / Abelson murine leukemia viral (v-abl) oncogene homolog 1 antibody / Abelson tyrosine-protein kinase 1 antibody / abl antibody / AS27_01606 antibody / AS28_01153 antibody / bcr /abl antibody / bcr /c-abl oncogene protein antibody / c-Abl antibody / c-ABL1 antibody / c-abl oncogene 1 / non-receptor tyrosine kinase antibody / c-abl oncogene 1 / receptor tyrosine kinase antibody / c-abl oncoprotein 1 / receptor tyrosine kinase antibody / c-abl protein / type I antibody / c-abl protein / type II antibody / c-abl protein / type III antibody / EH28_08971 antibody / H920_06685 antibody / hypothetical protein antibody / I79_022766 antibody / JTK7 antibody / M91_15851 antibody / M959_09404 antibody / MDA_GLEAN10011219 antibody / N300_09310 antibody / N301_05310 antibody / N302_13132 antibody / N303_06132 antibody / N305_08970 antibody / N306_14769 antibody / N307_02480 antibody / N308_09276 antibody / N310_02990 antibody / N311_05821 antibody / N312_13493 antibody / N320_05420 antibody / N321_09312 antibody / N322_03953 antibody / N324_04265 antibody / N325_05681 antibody / N326_05069 antibody / N327_00954 antibody / N328_09054 antibody / N329_05370 antibody / N330_00892 antibody / N331_05850 antibody / N332_01067 antibody / N333_04720 antibody / N334_08332 antibody / N335_09034 antibody / N336_02603 antibody / N339_12434 antibody / N340_00756 antibody / N341_05339 antibody / p150 antibody / PAL_GLEAN10012549 antibody / PANDA_003253 antibody / proto-oncogene c-Abl antibody / Proto-oncogene tyrosine-protein kinase ABL1 antibody / tyrosine-protein kinase ABL1 antibody / Tyrosine-protein kinase transforming protein Abl antibody / UY3_08252 antibody / v-abl antibody / v-abl Abelson murine leukemia oncogene 1 antibody / v-abl Abelson murine leukemia viral oncogene 1 antibody / v-abl Abelson murine leukemia viral oncogene homolog 1 antibody / v-abl Abelson murine leukemia viral oncogene-like 1 antibody / Y1Q_004344 antibody / Y956_02069 antibody / Z169_11685 antibody
Function Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates the caspase CASP9 on 'Tyr-153' and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. ABL1 acts also as a regulator of multiple pathological signaling cascades during infection. Several known tyrosine-phosphorylated microbial proteins have been identified as ABL1 substrates. This is the case of A36R of Vaccinia virus, Tir (translocated intimin receptor) of pathogenic E.coli and possibly Citrobacter, CagA (cytotoxin-associated gene A) of H.pylori, or AnkA (ankyrin repeat-containing protein A) of A.phagocytophilum. Pathogens can highjack ABL1 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.
Post-translational Modifications Acetylated at Lys-711 by EP300 which promotes the cytoplasmic translocation. ; Phosphorylation at Tyr-70 by members of the SRC family of kinases disrupts SH3 domain-based autoinhibitory interactions and intermolecular associations, such as that with ABI1, and also enhances kinase activity. Phosphorylation at Tyr-226 and Tyr-393 correlate with increased activity. DNA damage-induced activation of ABL1 requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation at Ser-569 has been attributed to a CDC2-associated kinase and is coupled to cell division (By similarity). Phosphorylation at Ser-618 and Ser-619 by PAK2 increases binding to CRK and reduces binding to ABI1. Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation. Phosphorylated by PRKDC (By similarity). ; Polyubiquitinated. Polyubiquitination of ABL1 leads to degradation.
Cellular Localization Cytoplasm, cytoskeleton. Nucleus. Mitochondrion Note=Shuttles between the nucleus and cytoplasm depending on environmental signals. Sequestered into the cytoplasm through interaction with 14-3-3 proteins. Localizes to mitochondria in response to oxidative stress (By similarity). ; Isoform IB: Nucleus membrane; Lipid-anchor. Note=The myristoylated c-ABL protein is reported to be nuclear.
Tissue Specificity Widely expressed.

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