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Anti-Phospho-Catenin-β (S37) antibody

[STJ90207] Download PDF Print Data Sheet

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Product name Anti-Phospho-Catenin-β (S37) antibody
Short Description Rabbit Polyclonal to Phospho-Catenin-β (S37).
Description Catenin-β is a protein encoded by the CTNNB1 gene which is approximately 85,4 kDa. Catenin-β is localised to the cytoplasm and nucleus. It is involved in CDK-mediated phosphorylation and removal of Cdc6, blood-brain barrier and immune cell transmigration, endometrial cancer and cell junction organization. It is part of a complex of proteins that constitute adherent junctions. Adherent junctions are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. It also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Catenin-β is expressed in several hair follicle cell types and the colon. Mutations in the CTNNB1 gene may result in colorectal cancer and pilomatrixoma. STJ90207 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous Catenin-β protein which only binds about S37 when S37 is phosphorylated.
Applications IHC-p, ELISA, IF, WB
Dilution range WB 1:500-1:2000 / IHC 1:100-1:300 / IF 1:200-1:1000 / ELISA 1:40000 /
Specificity Phospho-Catenin-β (S37) Polyclonal Antibody detects endogenous levels of Catenin-β protein only when phosphorylated at S37.
Protein Name Catenin beta-1 - CTNNB1 - beta-catenin - armadillo - CTNNB - catenin beta 1
Immunogen Synthesized peptide derived from human Catenin-β around the phosphorylation site of S37.
Immunogen Region 10-90 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 1499 (Human) / 12387 (Mouse) / 84353 (Rat)
Gene Symbol CTNNB1
Molecular Weight 85 kDa
Database Links Human UniProt/Swiss-Prot:P35222 Mouse UniPort/Swiss-Prot: Q02248 Rat UniProt/Swiss-Port: Q9WU82 Human Entrez Gene: 1499 Mouse Entrez Gene: 12387 Rat Entrez Gene: Rn.112601
Alternative Names CTNNB1 / catenin beta 1 / A306_10537 antibody / Anapl_11799 antibody / armadillo antibody / AS27_10434 antibody / AS28_06813 antibody / beta catenin antibody / beta-catenin antibody / beta catenin 1 antibody / beta-catenin 1 antibody / beta-catenin homologue antibody / beta-catenin protein antibody / Bfc antibody / catenin beta antibody / catenin beta1 antibody / Catenin beta-1 antibody / catenin beta 1 subunit antibody / catenin (cadherin associated protein) / beta 1 antibody / catenin (cadherin-associated protein) beta 1 antibody / catenin (cadherin-associated protein) / beta 1 antibody / catenin (cadherin associated protein) / beta 1 / 88kDa antibody / catenin (cadherin-associated protein) / beta 1 / 88kDa antibody / Catnb antibody / CB1_001546002 antibody / CHBCAT antibody / ctnb1 antibody / CTNNB antibody / D623_10018801 antibody / H920_05491 antibody / hypothetical protein antibody / Jup antibody / M91_15433 antibody / M959_12400 antibody / MDA_GLEAN10023294 antibody / Mesc antibody / MRD19 antibody / N300_01502 antibody / N301_11960 antibody / N302_16051 antibody / N303_02203 antibody / N305_10402 antibody / N306_00371 antibody / N307_01802 antibody / N308_03586 antibody / N309_13583 antibody / N310_00327 antibody / N311_03706 antibody / N312_02374 antibody / N320_12482 antibody / N321_11797 antibody / N322_08013 antibody / N324_12075 antibody / N325_04049 antibody / N326_09205 antibody / N327_05785 antibody / N328_12344 antibody / N329_12774 antibody / N330_06482 antibody / N331_01403 antibody / N332_06440 antibody / N333_05443 antibody / N334_08901 antibody / N335_02354 antibody / N336_06743 antibody / N339_09448 antibody / N340_05407 antibody / N341_07577 antibody / PANDA_013082 antibody / PsBeta-catenin antibody / UY3_11310 antibody / Y1Q_028147 antibody / Y956_11388 antibody / Z169_13134 antibody
Function Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML . Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity).
Post-translational Modifications Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription (By similarity). Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33. Phosphorylated by NEK2. EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. Phosphorylated on Ser-33 and Ser-37 by HIPK2 and GSK3B, this phosphorylation triggers proteasomal degradation . Phosphorylation on Ser-191 and Ser-246 by CDK5. Phosphorylation by CDK2 regulates insulin internalization. Phosphorylation by PTK6 at Tyr-64, Tyr-142, Tyr-331 and/or Tyr-333 with the predominant site at Tyr-64 is not essential for inhibition of transcriptional activity. ; Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation (By similarity). ; S-nitrosylation at Cys-619 within adherens junctions promotes VEGF-induced, NO-dependent endothelial cell permeability by disrupting interaction with E-cadherin, thus mediating disassembly adherens junctions. ; O-glycosylation at Ser-23 decreases nuclear localization and transcriptional activity, and increases localization to the plasma membrane and interaction with E-cadherin CDH1. ; Deacetylated at Lys-49 by SIRT1.
Cellular Localization Cytoplasm Nucleus Cytoplasm, cytoskeleton Cell junction, adherens junction Cell junction Cell membrane Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cell junction, synapse Note=Colocalized with RAPGEF2 and TJP1 at cell-cell contacts (By similarity). Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of beta-catenin is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. Colocalizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells. Interaction with FAM53B promotes translocation to the nucleus .
Tissue Specificity Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level).


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